Abstract: Through pot-experiment, we discussed the effect of cake fertilizer on some microelements of iron, copper, boron, manganese and zinc absorption in flue-cured tobacco middle leaves of K326. The results showed that the contents of iron, boron, manganese and zinc were highly significant difference between treatments A and E, B and E, C and E. There was no significant difference among the contents of all the five microelements. The contents of iron, boron, and manganese had the highest value on the forty leaf age. Zinc had the highest content on fifty leaf age, but it had little difference with the content of forty leaf age. The contents of iron and zinc were increased in the beginning, and then became lower. The contents of boron and manganese were increased in the beginning, then became lower, and then came to the highest, turn to lower at last. The content of copper had opposite change with these microelements. It reduced at beginning, then increased, and reduced at last.