Abstract: The biologic characters of pig were analyzed to acquire a new ERK6 gene in pig and provide information for its further study. Based on MAPK12 gene cDNA sequence of human and mouse, three pairs of primers were designed to amplify ORF of pig MAPK12 gene. The amino acid sequence was analyzed by bioinformatic methods and software. This ORF included 1101bp, encoding 367 amino acids, 92% similar to human and mouse. Its physical and chemical characters were analyzed and its primary and secondary structure was predicted. This isolate is named as pig ERK6 gene. Obtain of the enzyme’s basic information and contrast analysis of existing reports will play an important role on further study of its structure and function.