Abstract: Effects of Ca and B nutrient on quality and storage of longan fruit were discussed under the condition of lacking of boron nutrient in soil, and spraying three times on leaves and fruits with 1%CaCl2, 0.2%H3BO3, 1%CaCl2+0.2%H3BO3 solutions and water(CK) respectively during the developmental stage of longan aril. The results showed that both 0.2%H3BO3 and 1%CaCl2+0.2%H3BO3 spray had significantly increased B content, shape, weight, edible portion and sugar content in fruits. However, the effect of 0.2%H3BO3 treatment on quality was better than 1%CaCl2+0.2%H3BO3 treatment. Moreover, both the two treatments had increased healthy fruit rate, decreased fruit weight lose rate and inhibited changing speed of nutrition of flesh during storage. 1%CaCl2 spray had obviously increased content of Ca in fruits, defer postharvest senescence of fruit, but not significantly influenced on quality of longan fruit.