Chinese Agricultural Science Bulletin ›› 2024, Vol. 40 ›› Issue (6): 128-134.doi: 10.11924/j.issn.1000-6850.casb2023-0250
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JI Wei1,2(), SU Wenying1, LIU Xiaomei1, WANG Yipu1, REN Likai1(), XI Xiaoyan2, CHEN Kelong2
Received:
2023-03-23
Revised:
2023-05-15
Online:
2024-02-22
Published:
2024-02-22
JI Wei, SU Wenying, LIU Xiaomei, WANG Yipu, REN Likai, XI Xiaoyan, CHEN Kelong. Wild Ganoderma lingzhi Resources in Lianyungang Area: Genetic Diversity and Genetic Relationship Analysis Based on ISSR Markers[J]. Chinese Agricultural Science Bulletin, 2024, 40(6): 128-134.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2023-0250
菌种编号 | 形态特征 | 生境特点 |
---|---|---|
GL01 | 子实体无柄,呈红褐色,菌盖近半圆形、边缘肥厚、呈淡黄色,有轮纹 | 生于阔叶林中的立木干基部朽木上 |
GL02 | 子实体无柄,呈白色,菌盖边缘不规则、薄、呈淡黄褐色,有轮纹 | 生于阔叶林中地下枯树桩上 |
GL03 | 子实体无柄,呈红褐色,菌盖近圆形、边缘不规则、呈淡黄色,有轮纹 | 生于阔叶树腐木桩上 |
GL04 | 子实体有柄,呈红褐色,有漆样光泽,菌盖不规则、边缘薄、呈淡黄色 | 生于阔叶树倒木上 |
GL05 | 子实体有柄,呈红褐色,菌盖近似椭圆形,边缘不规则 | 生于阔叶林中地下腐木上 |
GL06 | 子实体为幼嫩阶段,呈近球形,黄色 | 生于阔叶林中的立木干基部枯树根上 |
GL07 | 子实体为幼嫩阶段,有两个分支,有漆样光泽,呈近球形,顶端呈淡黄色 | 生于阔叶树腐木桩上 |
GL08 | 子实体近扇形,白色,有轮纹 | 生于阔叶林中地下腐木上 |
GL09 | 子实体有柄,近扇形,红褐色,有轮纹 | 生于阔叶树朽板根上 |
GL10 | 子实体有柄,半圆形,暗红褐色,有轮纹 | 生于阔叶林中地下腐木上 |
GL11 | 子实体有柄,子实体为幼嫩阶段,扇形,呈黄色,边缘肥厚规则 | 生于阔叶林中地下腐木桩周围地上 |
GL12 | 子实体有短柄,子实体为幼嫩阶段,近半圆形,呈黄色,边缘肥厚 | 生于阔叶林中地下腐木木桩上 |
GL13 | 子实体有柄,呈红褐色,扇形,边缘肥厚规则 | 生于阔叶林中地下腐木桩周围地上 |
GL14 | 子实体有柄呈红褐色,近半圆形,边缘呈黄色,边缘肥薄、不规则 | 生于阔叶林中地下腐木木桩上 |
GL15 | 子实体有柄,呈红褐色,有漆样光泽,菌盖不规则、边缘厚 | 生于阔叶树倒木上 |
菌种编号 | 形态特征 | 生境特点 |
---|---|---|
GL01 | 子实体无柄,呈红褐色,菌盖近半圆形、边缘肥厚、呈淡黄色,有轮纹 | 生于阔叶林中的立木干基部朽木上 |
GL02 | 子实体无柄,呈白色,菌盖边缘不规则、薄、呈淡黄褐色,有轮纹 | 生于阔叶林中地下枯树桩上 |
GL03 | 子实体无柄,呈红褐色,菌盖近圆形、边缘不规则、呈淡黄色,有轮纹 | 生于阔叶树腐木桩上 |
GL04 | 子实体有柄,呈红褐色,有漆样光泽,菌盖不规则、边缘薄、呈淡黄色 | 生于阔叶树倒木上 |
GL05 | 子实体有柄,呈红褐色,菌盖近似椭圆形,边缘不规则 | 生于阔叶林中地下腐木上 |
GL06 | 子实体为幼嫩阶段,呈近球形,黄色 | 生于阔叶林中的立木干基部枯树根上 |
GL07 | 子实体为幼嫩阶段,有两个分支,有漆样光泽,呈近球形,顶端呈淡黄色 | 生于阔叶树腐木桩上 |
GL08 | 子实体近扇形,白色,有轮纹 | 生于阔叶林中地下腐木上 |
GL09 | 子实体有柄,近扇形,红褐色,有轮纹 | 生于阔叶树朽板根上 |
GL10 | 子实体有柄,半圆形,暗红褐色,有轮纹 | 生于阔叶林中地下腐木上 |
GL11 | 子实体有柄,子实体为幼嫩阶段,扇形,呈黄色,边缘肥厚规则 | 生于阔叶林中地下腐木桩周围地上 |
GL12 | 子实体有短柄,子实体为幼嫩阶段,近半圆形,呈黄色,边缘肥厚 | 生于阔叶林中地下腐木木桩上 |
GL13 | 子实体有柄,呈红褐色,扇形,边缘肥厚规则 | 生于阔叶林中地下腐木桩周围地上 |
GL14 | 子实体有柄呈红褐色,近半圆形,边缘呈黄色,边缘肥薄、不规则 | 生于阔叶林中地下腐木木桩上 |
GL15 | 子实体有柄,呈红褐色,有漆样光泽,菌盖不规则、边缘厚 | 生于阔叶树倒木上 |
引物编号 | 引物序列(5'-3') | 退火温度/℃ |
---|---|---|
UBC807 | AGAGAGAGAGAGAGAGT | 48 |
UBC809 | AGAGAGAGAGAGAGAGG | 45 |
UBC811 | GAGAGAGAGAGAGAGAC | 51 |
UBC836 | AGAGAGAGAGAGAGAGYA | 54 |
UBC840 | GAGAGAGAGAGAGAGAYT | 42 |
UBC841 | GAGAGAGAGAGAGAGAYC | 48 |
UBC842 | GAGAGAGAGAGAGAGAYG | 42 |
UBC880 | GGAGAGGAGAGGAGA | 45 |
UBC881 | GGGTGGGGTGGGGTG | 48 |
UBC888 | BDBCACACACACACACA | 42 |
引物编号 | 引物序列(5'-3') | 退火温度/℃ |
---|---|---|
UBC807 | AGAGAGAGAGAGAGAGT | 48 |
UBC809 | AGAGAGAGAGAGAGAGG | 45 |
UBC811 | GAGAGAGAGAGAGAGAC | 51 |
UBC836 | AGAGAGAGAGAGAGAGYA | 54 |
UBC840 | GAGAGAGAGAGAGAGAYT | 42 |
UBC841 | GAGAGAGAGAGAGAGAYC | 48 |
UBC842 | GAGAGAGAGAGAGAGAYG | 42 |
UBC880 | GGAGAGGAGAGGAGA | 45 |
UBC881 | GGGTGGGGTGGGGTG | 48 |
UBC888 | BDBCACACACACACACA | 42 |
引物编号 | 样本数 | 多态性位点 | 等位基因数 | 有效等位基因数(Ne) | Nei's遗传多样性(He) | Shannon信息指数(I) |
---|---|---|---|---|---|---|
UBC807 | 15 | 4 | 2 | 1.642 3 | 0.391 1 | 0.579 9 |
UBC809 | 15 | 6 | 2 | 1.142 1 | 0.124 4 | 0.244 9 |
UBC811 | 15 | 5 | 2 | 1.142 1 | 0.124 4 | 0.244 9 |
UBC836 | 15 | 1 | 2 | 1.142 1 | 0.124 4 | 0.244 9 |
UBC840 | 15 | 5 | 2 | 1.142 1 | 0.124 4 | 0.244 9 |
UBC841 | 15 | 3 | 1 | 1.000 0 | 0.103 1 | 0.223 5 |
UBC842 | 15 | 2 | 2 | 1.642 3 | 0.391 1 | 0.579 9 |
UBC880 | 15 | 5 | 2 | 1.300 6 | 0.231 1 | 0.392 7 |
UBC881 | 15 | 2 | 1 | 1.000 0 | 0.103 1 | 0.223 5 |
UBC888 | 15 | 7 | 2 | 1.300 6 | 0.231 1 | 0.392 7 |
平均 | 15 | 4 | 1.8 | 1.245 4 | 0.174 2 | 0.292 5 |
引物编号 | 样本数 | 多态性位点 | 等位基因数 | 有效等位基因数(Ne) | Nei's遗传多样性(He) | Shannon信息指数(I) |
---|---|---|---|---|---|---|
UBC807 | 15 | 4 | 2 | 1.642 3 | 0.391 1 | 0.579 9 |
UBC809 | 15 | 6 | 2 | 1.142 1 | 0.124 4 | 0.244 9 |
UBC811 | 15 | 5 | 2 | 1.142 1 | 0.124 4 | 0.244 9 |
UBC836 | 15 | 1 | 2 | 1.142 1 | 0.124 4 | 0.244 9 |
UBC840 | 15 | 5 | 2 | 1.142 1 | 0.124 4 | 0.244 9 |
UBC841 | 15 | 3 | 1 | 1.000 0 | 0.103 1 | 0.223 5 |
UBC842 | 15 | 2 | 2 | 1.642 3 | 0.391 1 | 0.579 9 |
UBC880 | 15 | 5 | 2 | 1.300 6 | 0.231 1 | 0.392 7 |
UBC881 | 15 | 2 | 1 | 1.000 0 | 0.103 1 | 0.223 5 |
UBC888 | 15 | 7 | 2 | 1.300 6 | 0.231 1 | 0.392 7 |
平均 | 15 | 4 | 1.8 | 1.245 4 | 0.174 2 | 0.292 5 |
菌株编号 | GL01 | GL02 | GL03 | GL04 | GL05 | GL06 | GL07 | GL08 | GL09 | GL10 | GL11 | GL12 | GL13 | GL14 | GL15 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
GL01 | 1.000 | ||||||||||||||
GL02 | 0.614 | 1.000 | |||||||||||||
GL03 | 0.722 | 0.664 | 1.000 | ||||||||||||
GL04 | 0.538 | 0.611 | 0.573 | 1.000 | |||||||||||
GL05 | 0.592 | 0.671 | 0.575 | 0.838 | 1.000 | ||||||||||
GL06 | 0.633 | 0.717 | 0.624 | 0.642 | 0.633 | 1.000 | |||||||||
GL07 | 0.527 | 0.571 | 0.502 | 0.677 | 0.716 | 0.512 | 1.000 | ||||||||
GL08 | 0.462 | 0.436 | 0.446 | 0.576 | 0.481 | 0.415 | 0.537 | 1.000 | |||||||
GL09 | 0.501 | 0.522 | 0.572 | 0.455 | 0.645 | 0.522 | 0.825 | 0.527 | 1.000 | ||||||
GL10 | 0.811 | 0.613 | 0.783 | 0.538 | 0.512 | 0.702 | 0.513 | 0.476 | 0.585 | 1.000 | |||||
GL11 | 0.657 | 0.747 | 0.661 | 0.542 | 0.514 | 0.814 | 0.561 | 0.457 | 0.596 | 0.717 | 1.000 | ||||
GL12 | 0.643 | 0.703 | 0.676 | 0.518 | 0.523 | 0.813 | 0.526 | 0.453 | 0.602 | 0.702 | 0.817 | 1.000 | |||
GL13 | 0.588 | 0.616 | 0.586 | 0.771 | 0.741 | 0.661 | 0.704 | 0.486 | 0.713 | 0.633 | 0.604 | 0.613 | 1.000 | ||
GL14 | 0.576 | 0.598 | 0.597 | 0.767 | 0.731 | 0.662 | 0.706 | 0.493 | 0.727 | 0.637 | 0.677 | 0.612 | 0.866 | 1.000 | |
GL15 | 0.596 | 0.612 | 0.582 | 0.838 | 0.817 | 0.621 | 0.671 | 0.482 | 0.712 | 0.603 | 0.670 | 0.614 | 0.717 | 0.740 | 1.000 |
菌株编号 | GL01 | GL02 | GL03 | GL04 | GL05 | GL06 | GL07 | GL08 | GL09 | GL10 | GL11 | GL12 | GL13 | GL14 | GL15 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
GL01 | 1.000 | ||||||||||||||
GL02 | 0.614 | 1.000 | |||||||||||||
GL03 | 0.722 | 0.664 | 1.000 | ||||||||||||
GL04 | 0.538 | 0.611 | 0.573 | 1.000 | |||||||||||
GL05 | 0.592 | 0.671 | 0.575 | 0.838 | 1.000 | ||||||||||
GL06 | 0.633 | 0.717 | 0.624 | 0.642 | 0.633 | 1.000 | |||||||||
GL07 | 0.527 | 0.571 | 0.502 | 0.677 | 0.716 | 0.512 | 1.000 | ||||||||
GL08 | 0.462 | 0.436 | 0.446 | 0.576 | 0.481 | 0.415 | 0.537 | 1.000 | |||||||
GL09 | 0.501 | 0.522 | 0.572 | 0.455 | 0.645 | 0.522 | 0.825 | 0.527 | 1.000 | ||||||
GL10 | 0.811 | 0.613 | 0.783 | 0.538 | 0.512 | 0.702 | 0.513 | 0.476 | 0.585 | 1.000 | |||||
GL11 | 0.657 | 0.747 | 0.661 | 0.542 | 0.514 | 0.814 | 0.561 | 0.457 | 0.596 | 0.717 | 1.000 | ||||
GL12 | 0.643 | 0.703 | 0.676 | 0.518 | 0.523 | 0.813 | 0.526 | 0.453 | 0.602 | 0.702 | 0.817 | 1.000 | |||
GL13 | 0.588 | 0.616 | 0.586 | 0.771 | 0.741 | 0.661 | 0.704 | 0.486 | 0.713 | 0.633 | 0.604 | 0.613 | 1.000 | ||
GL14 | 0.576 | 0.598 | 0.597 | 0.767 | 0.731 | 0.662 | 0.706 | 0.493 | 0.727 | 0.637 | 0.677 | 0.612 | 0.866 | 1.000 | |
GL15 | 0.596 | 0.612 | 0.582 | 0.838 | 0.817 | 0.621 | 0.671 | 0.482 | 0.712 | 0.603 | 0.670 | 0.614 | 0.717 | 0.740 | 1.000 |
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