Identification of Actinomyces 4-1 and Study on Bacteriostasis and Stability of Its Fermentation Broth

doi:10.11924/j.issn.1000-6850.casb2023-0135

Abstract

Abstract:

In order to explore the bacteria resources of saline-alkali soil and test their antibacterial function, actinomycete 4-1 was isolated and used as the original strain. Combined with morphological characteristics, physiological and biochemical indicators, as well as molecular biology techniques, the species status of 4-1 was comprehensively identified, while the antibacterial activity and stability of its fermentation broth were evaluated using agar diffusion method and mycelial growth rate inhibition method. The results showed that strain 4-1 showed the highest similarity to Streptomyces puniceus, with a homology of 99.93%, and the constructed phylogenetic tree showed that they were most closely related. The fermentation broth of strain 4-1 had a wide range of antibacterial spectrum and showed certain inhibitory effects on pathogenic bacteria (6 strains) and pathogenic fungi (5 strains). The stability of the fermentation broth was minimally affected by acidity, alkalinity, heat, or ultraviolet radiation. However, the antibacterial activity of the fermentation broth decreased when the temperature exceeded 90℃ or the pH was below 4. The availability and functional evaluation of this strain laid a foundation for further expanding rhizosphere growth-promoting bacteria resources and applications.

Key words: actinomyces, Streptomyces puniceus, strain identification, bacteriostasis, stability

GUO Yuhao, LIU Shaobo, GE Jingping, PING Wenxiang. Identification of Actinomyces 4-1 and Study on Bacteriostasis and Stability of Its Fermentation Broth[J]. Chinese Agricultural Science Bulletin, 2024, 40(9): 117-123.

Figures/Tables 7

References 30

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供试细菌	革兰氏染色	抑菌圈直径/mm
枯草芽孢杆菌Bacillus subtilis	阳性	15.5 ± 0.17 (＋＋＋)
金黄色葡萄球菌Staphylococcus aureus	阳性	16.5 ± 0.18 (＋＋＋)
蜡样芽胞杆菌Bacillus cereus	阳性	12 ± 0.45 (＋＋＋)
短小芽孢杆菌Bacillus pumilus	阳性	16 ± 0.20 (＋＋)
铜绿假单胞菌Pseudomonas aeruginosa	阴性	13 ± 0.34 (＋＋)
变形杆菌Proteus vulgaris	阴性	12 ± 0.50 (＋＋)
大肠杆菌Escherichia coil	阴性	0 (－)
肺炎克雷伯氏菌Klebsiella pneumoniae	阴性	0 (－)
阴沟肠杆菌Enterobacter cloacae	阴性	0 (－)

供试细菌	革兰氏染色	抑菌圈直径/mm
枯草芽孢杆菌Bacillus subtilis	阳性	15.5 ± 0.17 (＋＋＋)
金黄色葡萄球菌Staphylococcus aureus	阳性	16.5 ± 0.18 (＋＋＋)
蜡样芽胞杆菌Bacillus cereus	阳性	12 ± 0.45 (＋＋＋)
短小芽孢杆菌Bacillus pumilus	阳性	16 ± 0.20 (＋＋)
铜绿假单胞菌Pseudomonas aeruginosa	阴性	13 ± 0.34 (＋＋)
变形杆菌Proteus vulgaris	阴性	12 ± 0.50 (＋＋)
大肠杆菌Escherichia coil	阴性	0 (－)
肺炎克雷伯氏菌Klebsiella pneumoniae	阴性	0 (－)
阴沟肠杆菌Enterobacter cloacae	阴性	0 (－)

供试病原真菌	对照菌落直径/mm	处理菌落直径/mm	抑制率/%
小麦赤霉菌Gibberella zeae	85.0 ± 0.30	61.0 ± 0.17	29.41
禾谷镰刀菌Fusarium graminearum	60.0 ± 0.17	18.0 ± 0.17	70.00
辣椒根腐菌Phytophthora pepper	42.30 ± 0.20	37.65 ± 0.17	11.00
串珠镰刀菌Fusarium moniliforme sheld	74.50 ± 0.10	51.00 ± 0.17	31.54
瓜果腐霉菌Pythium melonii	84.50 ± 0.20	41.5 ± 0.17	50.90

供试病原真菌	对照菌落直径/mm	处理菌落直径/mm	抑制率/%
小麦赤霉菌Gibberella zeae	85.0 ± 0.30	61.0 ± 0.17	29.41
禾谷镰刀菌Fusarium graminearum	60.0 ± 0.17	18.0 ± 0.17	70.00
辣椒根腐菌Phytophthora pepper	42.30 ± 0.20	37.65 ± 0.17	11.00
串珠镰刀菌Fusarium moniliforme sheld	74.50 ± 0.10	51.00 ± 0.17	31.54
瓜果腐霉菌Pythium melonii	84.50 ± 0.20	41.5 ± 0.17	50.90