Differences in Inter-root Soil Metabolites of Ginseng Under Different Cultivation Patterns

doi:10.11924/j.issn.1000-6850.casb2023-0662

Abstract

Abstract:

The study aims to investigate the differences in inter-root soil metabolites between forest-grown ginseng (PSM) and ginseng grown on farmland (NPSM), and provide a scientific reference for improving the quality of forest-grown ginseng. The study employed GC-MS/MS assay and molecular biology analysis to compare the inter-root soil metabolites of the two types of ginseng. We detected a total of 68 metabolites in the inter-root soil of wild-like forest cultivated ginseng and farmland cultivated ginseng. Among these metabolites, 17 were significantly different, with 14 up-regulated and 3 down-regulated metabolites. The detected metabolites included sugars, lipids, organic acids, esters and other substances. Sugars accounted for 47% of the total significant differential metabolites, which was the largest category of differential metabolites. These different metabolites in rhizosphere soil could directly or indirectly affect the quality of forest ginseng. This study examined the differences in inter-root soil metabolites between forest-cultivated ginseng and farmland-cultivated ginseng. The findings provided a scientific reference for improving the quality of forest-cultivated ginseng and exploring the cultivation environment of forest ginseng to overcome the obstacles of ginseng continuous cropping, improving the medicinal and edible value of forest ginseng and to provide new ideas and methods for the rural revitalization strategy of the understory ginseng industry in the northeast region of China.

Key words: understory ginseng, inter-root soil, continuous crop barriers, metabolic differentials, farmland cultivation of ginseng, Gc-Ms/Ms, quality improvement

JIAO Jinlong, SHI Rui, WANG Yonggui, WANG Huiling, ZHANG Ao, YAN Xingru, WANG Shu, HE Xiahong. Differences in Inter-root Soil Metabolites of Ginseng Under Different Cultivation Patterns[J]. Chinese Agricultural Science Bulletin, 2024, 40(20): 46-53.

Figures/Tables 7

References 54

[1]	朱晓丽. 人参皂苷药理作用研究进展[J]. 中国药物经济学, 2017, 12(12):152-154.
[2]	李红艳, 赵雨, 张惠, 等. 人参蛋白对Hep-2细胞增殖影响的比较[J]. 中国民族民间药, 2010, 19(1):33-34.
[3]	李红艳, 赵雨, 孙晓迪, 等. 人参蛋白抗辐射损伤作用研究[J]. 时珍国医国药, 2010, 21(9):2143-2144.
[4]	李红艳, 赵雨, 孙晓迪, 等. 人参蛋白对小鼠免疫功能影响的研究[J]. 亚太传统医药, 2010, 6(1):14-16.
[5]	肖春萍, 杨利民, 马锋敏. 栽培年限对人参根际土壤微生物活性及微生物量的影响[J]. 中国中药杂志, 2014, 39(24):4740-4747.
[6]	WRIGHT C J, COLEMAN D C. Responses of soil microbial biomass, nematode trophic groups, N-mineralization, and litter decomposition to disturbance events in the southern appalachians[J]. Soil biology and biochemistry, 2002, 34(1):13-25.
[7]	赵莹, 杨克军, 赵长江, 等. 外源糖调控玉米光合系统和活性氧代谢缓解盐胁迫[J]. 中国农业科学, 2014, 47(20):3962-3972. doi: 10.3864/j.issn.0578-1752.2014.20.004
[8]	ZHAO B, DONG S T, ZHANG J W, et al. Effects of controlled-release fertiliser on nitrogen use efficiency in summer maize[J]. PLoS one, 2017, 8(8).
[9]	符建荣. 控释氮肥对水稻的增产效应及提高肥料利用率的研究[J]. 植物营养与肥料学报, 2001(2):145-152.
[10]	赵斌, 董树亭, 王空军, 等. 控释肥对夏玉米产量及田间氨挥发和氮素利用率的影响[J]. 应用生态学报, 2009, 20(11):2678-2684.
[11]	武志杰, 周健民. 我国缓释、控释肥料发展现状、趋势及对策[J]. 中国农业科技导报, 2001(1):73-76.
[12]	郭天财, 冯伟, 赵会杰, 等. 水分和氮素运筹对冬小麦生育后期光合特性及产量的影响[J]. 西北植物学报, 2003(9):1512-1517.
[13]	孟兆江, 卞新民, 刘安能, 等. 调亏灌溉对夏玉米光合生理特性的影响[J]. 水土保持学报, 2006(3):182-186.
[14]	戴俊英, 沈秀瑛, 徐世昌, 等. 水分胁迫对玉米光合性能及产量的影响[J]. 作物学报, 1995(3):356-363.
[15]	ROLLAND F, SHEEN J. Sugar sensing and signalling networks in plants[J]. Biochemical society transactions, 2005, 33(Pt 1).
[16]	ROLLAND F, BAENA-GONZALEZ E, SHEEN J. SUGAR sensing and signaling in plants: conserved and novel mechanisms[J]. Annual review of plant biology, 2006, 57(1):675-709.
[17]	刘宝光, 李成浩, 张含国. 红皮云杉胚性愈伤组织保持与增殖阶段影响因子的筛选与分析[J]. 东北林业大学学报, 2010, 38(7):56-60.
[18]	MISHRA B S, SINGH M, AGGRAWAL P, et al. Glucose and auxin signaling interaction in controlling arabidopsis thaliana seedlings root growth and development[J]. Plos one, 2009, 4(2):e4502.
[19]	SINGH M, GUPTA A, LAXMI A. Glucose control of root growth direction in Arabidopsis thaliana[J]. Journal of experimental botany, 2014, 65(12):2981-2993. doi: 10.1093/jxb/eru146 pmid: 24719453
[20]	KEUNEN E, PESHEV D, VANGRONSVELD J, et al. Plant sugars are crucial players in the oxidative challenge during abiotic stress: extending the traditional concept[J]. Plant cell and environment, 2013, 36:1242-1255.
[21]	BAENA-GONZA´LEZ E, SHEEN J. Convergent energy and stress signaling[J]. Plant cell, 2008, 12(9):1360-1385.
[22]	EVELAND A L, JACKSON D P. Sugars, signalling, and plant development[J]. Journal of experimental botany, 2012, 63:3367-3377. doi: 10.1093/jxb/err379 pmid: 22140246
[23]	胡梦芸, 李辉, 张颖君, 等. 水分胁迫下葡萄糖对小麦幼苗光合作用和相关生理特性的影响[J]. 作物学报, 2009, 35(4):724-732.
[24]	SIRINGAM K, JUNTAWONG N, CHAUM S, et al. Salt tolerance enhancement in indica rice (Oryza sativa L.) seedlings using exogenous sucrose supplementation[J]. Plant omics journal, 2012, 5(1):52-59.
[25]	SHEN H, YAN X. Exudation and accumulation of organic acidsin the roots of common bean (Phaseolus vulgaris L.) in response to low phosphorus and aluminum toxicity stress[J]. Acta ecologica sinica, 2002, 22(3):387-394.
[26]	HOFFLAND E, VAN DEN BOOGAARD R, NELEMANS J, et al. Biosynthesis and root exudation of citric and malicacid in phosphate-starved rape plants[J]. New phytologist, 1992, 122(4):675-680.
[27]	马云华, 王秀峰, 魏珉, 等. 黄瓜连作土壤酚酸类物质积累对土壤微生物和酶活性的影响[J]. 应用生态学报, 2005(11):145-149.
[28]	朱静平, 程凯. 3种水培植物根系分泌的有机酸对氮循环菌的影响[J]. 环境工程学报, 2011, 5(9):2139-2143.
[29]	秦丽. 间作系统中续断菊与作物Cd、Pb累积特征和根系分泌低分子有机酸机理[D]. 昆明: 云南农业大学, 2017.
[30]	周宝利, 李燕, 李东, 等. 化感物质松香酸对辣椒种子萌发,幼苗生长及根际微生物的影响[J]. 华北农学报, 2010, 25(5):155-160. doi: 10.7668/hbnxb.2010.05.032
[31]	宋亮, 潘开文, 王进闯, 等. 酚酸类物质对苜蓿种子萌发及抗氧化物酶活性的影响[J]. 生态学报, 2006(10):3393-3403.
[32]	李夏, 周宝利, 陈绍莉, 等. 茄子自毒物质对辣椒种子萌发及枯萎菌的化感效应[J]. 生态学报, 2009, 29(2):960-965.
[33]	吴强盛, 夏仁学, 邹英宁. 柑橘丛枝菌根真菌生长与根际有效磷和磷酸酶活性的相关性[J]. 应用生态学报, 2006(4):4685-4689.
[34]	ABBOTT L K, ROBSON A D, BOER D G. The effect of phos- phorus on the formation of hyphae in soil by the vesicular arbuscular mycorrhizal fungus[J]. New phytologist, 1984, 97:437-446.
[35]	贺学礼, 刘媞, 赵丽莉. 接种丛枝菌根对不同施氮水平下黄芪生理特性和营养成分的影响[J]. 应用生态学报, 2009, 20(9):2118-2122.
[36]	张永峰, 殷波. 玉米耐盐性研究进展[J]. 玉米科学, 2008, 16(6):83-85.
[37]	钮旭光, 陈其军, 王学臣. 植物体内肌醇磷脂代谢与渗透胁迫信号转导[J]. 中国农学通报, 2006(2):199-202.
[38]	袁红梅, 郭文栋, 赵丽娟, 等. 外源肌醇对玉米耐盐性的影响[J]. 黑龙江农业科学, 2014(1):17-20.
[39]	马琳. NaCl胁迫对牧草种子萌发与幼苗生理生化的影响及耐盐性评价[D]. 泰安: 山东农业大学, 2010.
[40]	QUINN A L, DALU A, MEEKER L S, et al. Effects of octamethyl- cyclotetrasiloxane (D4) on the luteinizing hormone (LH) surge and levels of various reproductive hormones in female sprague-dawley rats[J]. Reproductive toxicology, 2007, 23(4):532-540.
[41]	MEEKS R G, STUMP D G, SIDDIQUI W H, et al. An inhalation reproductive toxicity study ofoctamethylcyclotetrasiloxane (D4) in female rats using multiple and single day exposureregimens[J]. Reproductive toxicology, 2007, 23(2):192-201.
[42]	SIDDIQUI W H, STUMP D G, PLOTZKE K P, et al. A two-generation reproductive toxicity study ofoctamethylcyclotetrasiloxane (D4) in rats exposed by whole-body vapor inhalation[J]. Reproductive toxicology, 2007, 23(2):202-215.
[43]	MCKIM J M, WILGA P C, KOLESAR G B, et al. Evaluation of octamethyl- cyclotetrasiloxane (D4)as an inducer of rat hepatic microsomal cytochrome P450, UDP-glucuronosyltransferase, andepoxide hydrolase: A 28-day inhalation study[J]. Toxicological sciences, 1998, 41(1):29-41.
[44]	MCKIM J M, KOLESAR G B, JEAN P A, et al. Repeated inhalation exposure to Octamethylcyclotetrasiloxane produces Hepatomegaly, transient hepatic hyperplasia, and sustained hypertrophy in female fischer 344 Rats in a manner similar to Phenobarbital[J]. Toxicology & applied pharmacology, 2001, 172(2):83-92.
[45]	HE B, RHODES-BROWER S, MILLER M R, et al. Octamethylcyclotetrasiloxane exhibits estrogenic activity in mice via ERα[J]. Toxicology and applied pharmacology, 2003, 192(3):254-261.
[46]	QUINN ANNE L, REGAN JANE M, TOBIN JOSEPH M, et al. In vitro and in vivo evaluation of the estrogenic, androgenic, and progestagenic potential of two cyclic siloxanes[J]. Toxicological sciences: an official journal of the society of toxicology, 2007, 96(1).
[47]	MCKIM J M, WILGA P C, BRESLIN W J, et al. Potential estrogenic and antiestrogenic activity of the cyclic siloxane octamethylcyclotetrasiloxane (D4) and the linear siloxane hexamethyldisiloxane (HMDS) in immature rats using the uterotrophic assay[J]. Toxicological sciences: an official journal of the society of toxicology, 2001, 63(1).
[48]	XU S H, CHANDRA G. Fate of Cyclic Methylsiloxanes in Soils. 2. Rates of degradation and volatilization[J]. Environmental science & technology, 1999, 33(22).
[49]	XU S H. Fate of Cyclic Methylsiloxanes in Soils. 1. The Degradation pathway[J]. Environmental science & technology, 1999, 33(4).
[50]	程海涛, 张亚玉, 张连学, 等. 土壤环境与人参生长关系的研究进展[J]. 中药材, 2011, 34(2):313-317.
[51]	常敬礼, 杨德光, 谭巍巍, 等. 水分胁迫对玉米叶片光合作用的影响[J]. 东北农业大学学报, 2008, 39(11):1-5.
[52]	王晓娟, 贾志宽, 梁连友, 等. 不同有机肥量对旱地玉米光合特性和产量的影响[J]. 应用生态学报, 2012, 23(2):419-425.
[53]	段巍巍, 李慧玲, 肖凯, 等. 氮肥对玉米穗位叶光合作用及其生理生化特性的影响[J]. 华北农学报, 2007(1):26-29. doi: 10.3321/j.issn:1000-7091.2007.01.007
[54]	黄子纯. 典型区域环境中新型硅氧烷类化合物的识别方法及其生成/降解机制[D]. 武汉: 江汉大学, 2020.