Paenibacillus polymyxa Identification and Its Antibacterial Effect on Pyricularia oryzae

doi:10.11924/j.issn.1000-6850.casb2023-0723

Abstract

Abstract:

In order to broaden the resources of microbial biocontrol agents and to develop biocontrol strains with high efficacy in the control of Pyricularia oryzae, the present study was conducted to screen a strain KH-19 with a good antagonistic effect from the inter-root soil of rice. The strain was identified by morphological observation, physiological and biochemical tests, and 16S rDNA sequencing. The inhibitory activity was determined by the oxford cup method and the plate stand-off method. The effectiveness of the strain was tested against P. oryzae in pots. The results showed that the KH-19 strain was Paenibacillus polymyxa, which had bacteriostatic effects on three strains of Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Pectobacterium carotovorum BC2, and also had good antagonistic effects on other six pathogenic bacteria and eight pathogenic fungi, and its inhibition rate reached 63.67% against P. oryzae. The results of the pot test showed that the disease index of P. oryzae decreased with the increase of the concentration of P. polymyxa KH-19, and the disease index of P. polymyxa on the 20th day of inoculation reached 78.11% when the concentration of the bacterial suspension was 10⁶-10⁷ cfu/mL. The results showed that P. polymyxa KH-19 had potential application value as a biocontrol strain with broad-spectrum inhibitory activity against P. oryzae.

Key words: Paenibacillus polymyxa, strain identification, pot experiment, Pyricularia oryzae, rice disease control, biocontrol strain, Paenibacillus polymyxa, antimicrobial activity

DOU Longtao, QU Xiaojun, HU Jihua, JIANG Wei, TIAN Yuan, YAN Gengxuan, ZHANG Shumei. Paenibacillus polymyxa Identification and Its Antibacterial Effect on Pyricularia oryzae[J]. Chinese Agricultural Science Bulletin, 2024, 40(23): 118-125.

Figures/Tables 9

References 41

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项目	结果	文献	项目	结果	文献	项目	结果	文献
柠檬酸盐利用	+	[21]	淀粉水解	+	[22]	甲基红试验	-	[23]
明胶液化	+	[21]	硝酸盐还原	+	[22]	0% NaCl	+	*
吲哚反应	+	[21]	葡萄糖氧化发酵	+	[22]	2% NaCl	+	*
甘露醇发酵	+	[21]	丙三醇发酵	+	[22]	5% NaCl	+	*
蔗糖发酵	+	[21]	乳糖发酵	+	[22]	7% NaCl	-	*
V-P 试验	+	[22]	接触酶反应	+	[22]	10% NaCl	-	*

项目	结果	文献	项目	结果	文献	项目	结果	文献
柠檬酸盐利用	+	[21]	淀粉水解	+	[22]	甲基红试验	-	[23]
明胶液化	+	[21]	硝酸盐还原	+	[22]	0% NaCl	+	*
吲哚反应	+	[21]	葡萄糖氧化发酵	+	[22]	2% NaCl	+	*
甘露醇发酵	+	[21]	丙三醇发酵	+	[22]	5% NaCl	+	*
蔗糖发酵	+	[21]	乳糖发酵	+	[22]	7% NaCl	-	*
V-P 试验	+	[22]	接触酶反应	+	[22]	10% NaCl	-	*

处理组	接种水稻稻病菌10 d		接种水稻稻病菌15 d		接种水稻稻病菌20 d		接种水稻稻病菌25 d
处理组	病情指数	防病效果/%	病情指数	防病效果/%	病情指数	防病效果/%	病情指数	防病效果/%
处理一	13.80	-	35.34	-	68.44	-	83.12	-
处理二	4.79	56.28	7.21	63.07	14.86	78.11	29.11	60..39
处理三	7.76	26.51	15.27	38.91	22.81	65.90	41.85	55.19
处理四	10.23	18.69	20.11	31.59	40.37	49.61	56.34	38.23

处理组	接种水稻稻病菌10 d		接种水稻稻病菌15 d		接种水稻稻病菌20 d		接种水稻稻病菌25 d
处理组	病情指数	防病效果/%	病情指数	防病效果/%	病情指数	防病效果/%	病情指数	防病效果/%
处理一	13.80	-	35.34	-	68.44	-	83.12	-
处理二	4.79	56.28	7.21	63.07	14.86	78.11	29.11	60..39
处理三	7.76	26.51	15.27	38.91	22.81	65.90	41.85	55.19
处理四	10.23	18.69	20.11	31.59	40.37	49.61	56.34	38.23