Lignocellulose Degradation Strains with High-temperature Resistance: Isolation, Screening, Identification and Degradation Process

doi:10.11924/j.issn.1000-6850.casb20190600244

Abstract

Abstract:

This study aims at screening out the bacterial strains with higher lignocellulolytic degradation enzyme activity under high temperature, and to explore the characteristics of straw lignocellulose degradation by bacterial strain. Soil samples were collected around forest hot spring in Mount Taibai and were used for enrichment culture at high temperature. The target strains were selected by decolorization circle method and colorimetric method. The types of strains were identified by morphological observation and 16S rDNA sequence analysis. The activities of cellulase, laccase, lignin peroxidase and manganese peroxidase were determined. The results showed that the cellulase activity of X-08 strain was 0.02872 U/mL, and the activity of MnP, LiP and Lac in M-17 strain was 51 U/mL, 672 U/mL and 192 U/mL, respectively. The X-08 strain was identified as Anoxybacillus rupiensis and M-17 strain was Geobacillus thermocatenulatus. With double bacteria degradation, the degradation rate of lignin and cellulose was 24.51% and 20.47% respectively after 20 days. The results could provide new bacterial resources for the biodegradation of agricultural waste, and a new idea for the degradation of lignocellulose in straw.

Key words: thermotolerant bacteria, decompose lignocellulose, screening, strain identification, degradation process

CLC Number:

S182

Liu Xinwu, Zhang Wei, Ma Lingling, Lv Xin. Lignocellulose Degradation Strains with High-temperature Resistance: Isolation, Screening, Identification and Degradation Process[J]. Chinese Agricultural Science Bulletin, 2020, 36(21): 118-125.

Figures/Tables 10

References 32

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试验组	接种菌株	降解处理天数/d
A1	X-08	4
A2	X-08	8
A3	X-08	12
A4	X-08	16
A5	X-08	20
B1	M-17	4
B2	M-17	8
B3	M-17	12
B4	M-17	16
B5	M-17	20
C1	M-17降解处理10天后接种菌X-08	20
C2	X-08降解处理10天后接种菌M-17	20
D1	未灭菌未接种菌株	20
D2	灭菌后未接种菌株	20

试验组	接种菌株	降解处理天数/d
A1	X-08	4
A2	X-08	8
A3	X-08	12
A4	X-08	16
A5	X-08	20
B1	M-17	4
B2	M-17	8
B3	M-17	12
B4	M-17	16
B5	M-17	20
C1	M-17降解处理10天后接种菌X-08	20
C2	X-08降解处理10天后接种菌M-17	20
D1	未灭菌未接种菌株	20
D2	灭菌后未接种菌株	20

菌株	酶活力/(U/mL)	菌株	酶活力/(U/mL)
X-010	0.02106	X-5	0.02523
X-08	0.02872	X-06	0.01966
X-015	0.02663	X-8	0.02315

菌株	酶活力/(U/mL)	菌株	酶活力/(U/mL)
X-010	0.02106	X-5	0.02523
X-08	0.02872	X-06	0.01966
X-015	0.02663	X-8	0.02315