Bacillus subtilis Against Tomato Fusarium Wilt: ARTP Mutagenesis and Screening

doi:10.11924/j.issn.1000-6850.casb20190800545

Abstract

Abstract:

Atmospheric and room temperature plasma (ARTP) technique was used to obtain mutants with enhanced antagonistic activity against tomato fusarium wilt. Bacillus subtilis N which had antagonistic effect on tomato fusarium wilt was chosen as the initial strain. Detection of inhibition zone was carried out to screen mutants and the control effect of the positive strains against tomato fusarium wilt was investigated via pot experiment. Five positive mutants were obtained through antagonistic zone screening and second screening of inhibition zone, in which the strain 62 showed the highest positive-mutation range, the diameter of inhibition zone was increased by 31.43% compared with the original strain. After 6 times of consecutive subculturing, the strain 62 showed good genetic stability. In the pot experiment, the prevention effect of the strain 62 on tomato fusarium wilt reached 75.76%. The selected strain 62 treated by ARTP is genetically stable, and shows significantly improved antagonistic activity, thus it is believed that this strain has a great application potential in the control of tomato fusarium wilt.

Key words: mutagenesis breeding, Bacillus subtilis, tomato fusarium wilt, atmospheric and room temperature plasma (ARTP), control effect of pot experiment

CLC Number:

S435.79

Zhang Xinqing, Zhang Xiaoxiao, Yang Chuanlun, Guo Nannan, Ma Nana, Cai Qianqian, Wang Xiuzhi. Bacillus subtilis Against Tomato Fusarium Wilt: ARTP Mutagenesis and Screening[J]. Chinese Agricultural Science Bulletin, 2020, 36(26): 44-49.

Figures/Tables 7

References 20

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批次	分离菌株数	正突变菌株数	正突变率/%
1	100	34	34.00
2	100	37	37.00
3	150	59	39.33
4	150	60	40.00
5	144	54	37.50

批次	分离菌株数	正突变菌株数	正突变率/%
1	100	34	34.00
2	100	37	37.00
3	150	59	39.33
4	150	60	40.00
5	144	54	37.50

序号	正突变幅度/%	序号	正突变幅度/%	序号	正突变幅度/%	序号	正突变幅度/%
5	1.33±0.11	59	8.67±0.15	117	0.79±0.08	179	0.02±0.01
6	4.00±0.21	60	6.43±0.42	119	1.33±0.14	180	7.83±0.21
7	2.00±0.06	61	12.08±0.70	122	0.67±0.01	181	4.70±0.13
8	4.00±0.10	^?62	31.43±0.27	124	3.30±0.02	184	6.27±0.21
13	1.33±0.15	64	5.63±0.04	126	10.54±0.08	^?190	16.42±0.25
16	0.67±0.15	65	5.63±0.17	128	11.20±0.14	^?191	16.42±0.07
18	0.67±0.06	66	7.24±0.10	130	0.67±0.07	192	7.83±0.11
25	3.33±0.15	67	12.08±0.70	131	0.01±0.14	193	4.70±0.07
26	4.00±0.07	68	1.60±0.00	135	13.83±0.11	197	8.61±0.05
27	10.00±0.10	69	3.21±0.03	136	13.83±0.14	198	7.05±0.02
28	11.33±0.15	70	2.40±0.04	^?137	21.07±0.16	199	10.17±0.12
33	2.67±0.06	71	5.63±0.04	138	9.88±0.02	201	2.36±0.01
34	4.00±0.09	72	1.60±0.00	139	2.65±0.05	202	7.05±0.00
35	0.67±0.15	74	3.21±0.09	140	0.01±0.00	204	9.39±0.06
36	1.33±0.03	76	6.43±2.42	143	2.65±0.00	205	4.70±0.02
37	10.00±0.20	77	8.85±0.00	144	1.99±0.07	206	9.39±0.03
38	0.67±0.05	78	7.24±0.40	145	1.33±0.04	207	6.27±0.04
39	3.33±0.15	79	8.05±0.29	152	0.01±0.00	208	5.49±0.03
40	12.67±0.15	80	3.21±0.12	156	1.99±0.02	209	8.61±0.21
41	1.33±0.15	82	3.21±0.09	159	7.91±0.06	210	1.58±0.00
42	0.67±0.05	83	1.60±0.02	160	6.59±0.04	213	9.39±0.02
43	0.67±1.15	85	2.40±0.14	161	7.25±0.02	214	0.02±0.00
45	12.00±0.00	86	2.40±0.01	162	1.33±0.06	217	0.80±0.02
46	5.33±0.11	88	0.79±0.00	163	3.30±0.11	220	1.58±0.00
47	11.73±0.46	91	0.79±0.00	164	3.96±0.08	222	10.17±0.04
48	11.33±0.15	92	1.60±0.00	165	1.33±0.06	223	4.70±0.11
49	1.33±0.31	93	9.66±0.24	174	2.65±0.09	224	4.70±0.04
^?51	18.00±0.20	94	8.85±0.28	175	7.91±0.06	225	8.61±0.03
55	2.67±0.06	96	1.60±0.01	176	1.33±0.00	227	0.02±0.00
57	1.33±0.31	97	0.79±0.00	178	4.70±0.06	230	3.14±0.01

序号	正突变幅度/%	序号	正突变幅度/%	序号	正突变幅度/%	序号	正突变幅度/%
5	1.33±0.11	59	8.67±0.15	117	0.79±0.08	179	0.02±0.01
6	4.00±0.21	60	6.43±0.42	119	1.33±0.14	180	7.83±0.21
7	2.00±0.06	61	12.08±0.70	122	0.67±0.01	181	4.70±0.13
8	4.00±0.10	^?62	31.43±0.27	124	3.30±0.02	184	6.27±0.21
13	1.33±0.15	64	5.63±0.04	126	10.54±0.08	^?190	16.42±0.25
16	0.67±0.15	65	5.63±0.17	128	11.20±0.14	^?191	16.42±0.07
18	0.67±0.06	66	7.24±0.10	130	0.67±0.07	192	7.83±0.11
25	3.33±0.15	67	12.08±0.70	131	0.01±0.14	193	4.70±0.07
26	4.00±0.07	68	1.60±0.00	135	13.83±0.11	197	8.61±0.05
27	10.00±0.10	69	3.21±0.03	136	13.83±0.14	198	7.05±0.02
28	11.33±0.15	70	2.40±0.04	^?137	21.07±0.16	199	10.17±0.12
33	2.67±0.06	71	5.63±0.04	138	9.88±0.02	201	2.36±0.01
34	4.00±0.09	72	1.60±0.00	139	2.65±0.05	202	7.05±0.00
35	0.67±0.15	74	3.21±0.09	140	0.01±0.00	204	9.39±0.06
36	1.33±0.03	76	6.43±2.42	143	2.65±0.00	205	4.70±0.02
37	10.00±0.20	77	8.85±0.00	144	1.99±0.07	206	9.39±0.03
38	0.67±0.05	78	7.24±0.40	145	1.33±0.04	207	6.27±0.04
39	3.33±0.15	79	8.05±0.29	152	0.01±0.00	208	5.49±0.03
40	12.67±0.15	80	3.21±0.12	156	1.99±0.02	209	8.61±0.21
41	1.33±0.15	82	3.21±0.09	159	7.91±0.06	210	1.58±0.00
42	0.67±0.05	83	1.60±0.02	160	6.59±0.04	213	9.39±0.02
43	0.67±1.15	85	2.40±0.14	161	7.25±0.02	214	0.02±0.00
45	12.00±0.00	86	2.40±0.01	162	1.33±0.06	217	0.80±0.02
46	5.33±0.11	88	0.79±0.00	163	3.30±0.11	220	1.58±0.00
47	11.73±0.46	91	0.79±0.00	164	3.96±0.08	222	10.17±0.04
48	11.33±0.15	92	1.60±0.00	165	1.33±0.06	223	4.70±0.11
49	1.33±0.31	93	9.66±0.24	174	2.65±0.09	224	4.70±0.04
^?51	18.00±0.20	94	8.85±0.28	175	7.91±0.06	225	8.61±0.03
55	2.67±0.06	96	1.60±0.01	176	1.33±0.00	227	0.02±0.00
57	1.33±0.31	97	0.79±0.00	178	4.70±0.06	230	3.14±0.01

处理组	发病率/%	病情指数/%	防效/%
空白对照	100.00^a	62.50^a	/
药剂对照	80.00^b	18.97^b	69.65^a
菌剂组	60.00^c	15.15^c	75.76^b