Isolation and Identification of Microalgae and Screening of Energy Algae Species in Alpine Region of Heilongjiang Province

doi:10.11924/j.issn.1000-6850.casb20190700464

Abstract

Abstract:

The objectives are to obtain an excellent strain of algae with high oil production, and explore the growth and oil production of different algae strains. In this study, water samples were collected from lakes in Daqing area of Heilongjiang Province. A total of 8 algae strains were isolated and screened. Species identification of algal strains was carried out by morphological and molecular biological methods. At the same time, the relationship between the algae species and the physical and chemical factors of the lake was analyzed by RDA. The growth, lipid synthesis and by-product content of the algae strain were studied. The results showed that the biomass of HDL01, HDL04, HDL05 and HDL07 were positively correlated with some environmental factors. The biomass growth rate and lipid growth rate of Monoraphidium convolutum HDL08 were second only to that of HDL01, which was 0.077±0.006 g/(L·d) and 5.994±0.003 mg/(L·d), respectively. Combined with the analysis of lipid composition and by-product content of algal strains, it was found that HDL08 had the potential to produce biodiesel for the test algae strain, which provided a certain support for enriching Chinese algae resources and using microalgae to produce biodiesel.

Key words: Monoraphidium convolutum, isolation, identification, lipid, astaxanthin, carotenoids

CLC Number:

S182

Zhou Yuhang, Li Qi, He Ping, Ge Jingping, Ping Wenxiang, Ling Hongzhi. Isolation and Identification of Microalgae and Screening of Energy Algae Species in Alpine Region of Heilongjiang Province[J]. Chinese Agricultural Science Bulletin, 2020, 36(26): 33-43.

Figures/Tables 16

References 27

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参数	兰德湖	滨洲湖	萨北湖	黎明湖
经纬度	46.64°N 125.13°E	46.62°N 125.15°E	46.67°N 125.13°E	46.59°N 125.14°E
电导率/(μS/cm)	1208	397	1347	413
TDS/(g/L)	35.2	11.58	39.2	12.05
盐度/%	0.06	0.02	0.08	0.02
pH	7.0~7.5	6.5~7.0	8.0~8.5	6.5~7.0
温度/℃	20.9	21	20.5	21
色度	10	4	15	4.3
磷/(mg/L)	0.147	0.04	0.08	0.03
总氮/(mg/L)	1.34	0.64	2.91	0.854
亚硝基氮/(μg/L)	0.571	1.428	29.428	0.285
氨氮/(mg/L)	0.754	0.38	1.36	0.152
溶解氧/(mg/L)	3.4	2.96	2.7	3.18
有机质/(mg/L)	14.46	9.66	31.52	10.42
Fe³⁺/(μg/L)	0.027	0.173	0.017	0.004
Mg²⁺/(μg/L)	14.144	6.476	14.902	9.96
Mn²⁺/(μg/L)	0.024	0	0.053	0.032
Ca²⁺/(μg/L)	161.03	228.375	137.825	195.08

参数	兰德湖	滨洲湖	萨北湖	黎明湖
经纬度	46.64°N 125.13°E	46.62°N 125.15°E	46.67°N 125.13°E	46.59°N 125.14°E
电导率/(μS/cm)	1208	397	1347	413
TDS/(g/L)	35.2	11.58	39.2	12.05
盐度/%	0.06	0.02	0.08	0.02
pH	7.0~7.5	6.5~7.0	8.0~8.5	6.5~7.0
温度/℃	20.9	21	20.5	21
色度	10	4	15	4.3
磷/(mg/L)	0.147	0.04	0.08	0.03
总氮/(mg/L)	1.34	0.64	2.91	0.854
亚硝基氮/(μg/L)	0.571	1.428	29.428	0.285
氨氮/(mg/L)	0.754	0.38	1.36	0.152
溶解氧/(mg/L)	3.4	2.96	2.7	3.18
有机质/(mg/L)	14.46	9.66	31.52	10.42
Fe³⁺/(μg/L)	0.027	0.173	0.017	0.004
Mg²⁺/(μg/L)	14.144	6.476	14.902	9.96
Mn²⁺/(μg/L)	0.024	0	0.053	0.032
Ca²⁺/(μg/L)	161.03	228.375	137.825	195.08

编号	采样点	名称	细胞大小	细胞形态	培养特征
HDL01	黎明湖	小球藻	直径3~5 μm	单细胞圆形	绿色,细胞不易沉降
HDL02	滨洲湖	微芒藻	直径4~8 μm	单细胞圆形	绿色,细胞不易沉降
HDL03	黎明湖	微芒藻	直径4~8 μm	单细胞圆形	深绿色,细胞不易沉降
HDL04	萨北湖	栅列藻	宽4~6 μm,长10~15 μm	2×4×长形细胞排列有鞭毛	深绿色,细胞不易沉降
HDL05	滨洲湖	微芒藻	直径5~7 μm	单细胞圆形	绿色,细胞不易沉降
HDL06	兰德湖	栅列藻	宽4~6 μm,长8~12 μm	2×4×长形细胞排列有鞭毛	深绿色,细胞不易沉降
HDL07	黎明湖	栅列藻	宽2~5 μm,长5~7 μm	两细胞聚集	黄绿色,细胞不易沉降
HDL08	萨北湖	单针藻	宽2~4 μm,长5~7 μm	单细胞月牙形	深绿色,细胞不易沉降

编号	采样点	名称	细胞大小	细胞形态	培养特征
HDL01	黎明湖	小球藻	直径3~5 μm	单细胞圆形	绿色,细胞不易沉降
HDL02	滨洲湖	微芒藻	直径4~8 μm	单细胞圆形	绿色,细胞不易沉降
HDL03	黎明湖	微芒藻	直径4~8 μm	单细胞圆形	深绿色,细胞不易沉降
HDL04	萨北湖	栅列藻	宽4~6 μm,长10~15 μm	2×4×长形细胞排列有鞭毛	深绿色,细胞不易沉降
HDL05	滨洲湖	微芒藻	直径5~7 μm	单细胞圆形	绿色,细胞不易沉降
HDL06	兰德湖	栅列藻	宽4~6 μm,长8~12 μm	2×4×长形细胞排列有鞭毛	深绿色,细胞不易沉降
HDL07	黎明湖	栅列藻	宽2~5 μm,长5~7 μm	两细胞聚集	黄绿色,细胞不易沉降
HDL08	萨北湖	单针藻	宽2~4 μm,长5~7 μm	单细胞月牙形	深绿色,细胞不易沉降

藻株编号	比生长速率(/d)	生物量/(g/L)	生物量增长率/[g/(L·d)]	含油量/(mg/g)	脂质增长率/[mg/(L·d)]
HDL01	0.153±0.001a	0.585±0.044b	0.090±0.007a	12.787±0.641a	6.440±0.003a
HDL02	0.087±0.007de	0.619±0.029ab	0.054±0.003c	7.524±0.588e	3.787±0.002g
HDL03	0.097±0.018d	0.515±0.024c	0.050±0.011cd	10.604±0.433c	5.325±0.006d
HDL04	0.078±0.001e	0.261±0.025e	0.020±0.002f	8.949±0.072d	4.485±0.001e
HDL05	0.094±0.007d	0.464±0.025d	0.044±0.001de	11.215±0.177bc	5.632±0.001c
HDL06	0.114±0.002c	0.193±0.028f	0.022±0.003f	7.455±0.346e	3.741±0.001h
HDL07	0.133±0.005b	0.275±0.021e	0.037±0.002e	8.370±0.127de	4.203±0.001f
HDL08	0.121±0.005bc	0.641±0.026a	0.077±0.006b	11.910±0.509ab	5.994±0.003b