Lipid-producing Monoraphidium sp.: The Best Medium Screening and Lipid-producing Property Enhancement

doi:10.11924/j.issn.1000-6850.casb2022-0048

Abstract

Abstract:

To investigate the effects of medium type and composition on the lipid-producing properties of Monoraphidium sp. HDMA-11 and fully improve the lipid production potential of the strain, the growth, biomass and lipid content of HDMA-11 were examined using cell counting, dry cell weight and Nile red staining methods in BG-11, BBM, TAP, D1 and SE media. The type and concentration of carbon and nitrogen sources were then optimized to improve the lipid production characteristics of the strain. Results showed that the TAP medium could greatly shorten the culture period of the strain and increase its biomass productivity by 127% and lipid productivity by 117%. The lipid-producing indexes of the strain were further improved when 2 mL/L of acetic acid and 750 mg/L of ammonium chloride were added. The productivity of biomass and lipid of the strain after being improved were 70 mg/(L·d) and 315 RFU/(L·d) respectively, which was 218% and 116% higher than those of the initial levels. This work optimized the culture conditions of HDMA-11, elucidated the effects of acetic acid and ammonium chloride on the growth and lipid-producing indexes, and provided data basis for further exploration of the lipid-producing potential of the strain.

Key words: Monoraphidium sp., medium, lipid-producing properties, biomass, lipid content

CLC Number:

S917

MA Siqi, LI Chang, FAN Yang, SUN Ying, YIN Ziliang, LIN Yimeng, GE Jingping. Lipid-producing Monoraphidium sp.: The Best Medium Screening and Lipid-producing Property Enhancement[J]. Chinese Agricultural Science Bulletin, 2022, 38(27): 157-164.

Figures/Tables 8

References 26

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培养基		生物量/(mg/L)	生物量生产率/[mg/(L·d)]	油脂含量/RFU	油脂生产率/[RFU/(L·d)]
BG-11		490	22	5.3	118
TAP	优化前	301	50	5.1	256
TAP	优化后	419	70	4.5	314

培养基		生物量/(mg/L)	生物量生产率/[mg/(L·d)]	油脂含量/RFU	油脂生产率/[RFU/(L·d)]
BG-11		490	22	5.3	118
TAP	优化前	301	50	5.1	256
TAP	优化后	419	70	4.5	314