The Biocontrol Strain GDND-2 of Tobacco Fusarium Root Rot: Identification and Control Effect

doi:10.11924/j.issn.1000-6850.casb2022-0746

Abstract

Abstract:

The study was to reserve resources for the development of new biocontrol agents to control tobacco Fusarium root rot. The antagonistic strains were screened against Fusarium oxysporum, and were identified throgh morphological, biochemical characteristics and molecular technique. The tolerance of the antagonistic strain was determined by plate scribing method, and its control effect on tobacco root rot was verified by potting experiment. The antagonistic strain GDND-2 was screened. Its fermentation filtrate had a strong inhibitory effect on mycelium growth of F. oxysporium, the inhibition rates of 10% and 20% fermentation filtrate were 53.09% and 62.24%. The strain GDND-2 was identified as Bacillus velenzensis. It presented a broad inhibitory spectrum, and showed strong tolerance to extreme temperature, acid-alkali and salt. It was not sensitive to seven commonly used fungicides such as carbendazim. The control effects of fermentation broth of GDND-2 on tobacco root rot were 70.00%, 74.62% and 79.51% on the 15^th, 30^th and 45^th day after root drenching, and its control effect on the 45^th day was higher than carbendazim. Bacillus velenzensis GDND-2 with excellent traits and broad inhibitory spectrum could effectively control tobacco Fusarium root rot, and it is expected to be developed as a biocontrol agent.

Key words: tobacco Fusarium root rot, Bacillus velenzensis, identification, tolerance, control effect

SHEN Huifang, YANG Qiyun, ZHANG Jingxin, PU Xiaoming, SUN Dayuan, LIU Pingping, LIN Birun. The Biocontrol Strain GDND-2 of Tobacco Fusarium Root Rot: Identification and Control Effect[J]. Chinese Agricultural Science Bulletin, 2024, 40(23): 126-134.

Figures/Tables 10

References 36

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菌株编号	抑菌带宽度/mm	10%发酵滤液		20%发酵滤液
菌株编号	抑菌带宽度/mm	菌丝直径/mm	抑制率/%	菌丝直径/mm	抑制率/%
GDND-2	9.97±0.17a	34.57±1.05e	53.09	27.83±0.97e	62.24
GDNX-5	7.37±0.12c	57.47b±2.04	22.09	47.17±0.74b	36.00
GDWH-8	8.50±0.22b	40.83±1.49d	44.60	32.43±0.87d	56.00
GDPY-2	7.20±0.14c	51.90±0.92c	29.58	42.13±1.09c	42.84
CK		73.70±1.61a		73.70±1.61a

菌株编号	抑菌带宽度/mm	10%发酵滤液		20%发酵滤液
菌株编号	抑菌带宽度/mm	菌丝直径/mm	抑制率/%	菌丝直径/mm	抑制率/%
GDND-2	9.97±0.17a	34.57±1.05e	53.09	27.83±0.97e	62.24
GDNX-5	7.37±0.12c	57.47b±2.04	22.09	47.17±0.74b	36.00
GDWH-8	8.50±0.22b	40.83±1.49d	44.60	32.43±0.87d	56.00
GDPY-2	7.20±0.14c	51.90±0.92c	29.58	42.13±1.09c	42.84
CK		73.70±1.61a		73.70±1.61a

生理生化试验	结果	生理生化试验	结果	生理生化试验	结果
接触酶	+	酪蛋白水解	+	蜜二糖产酸	-
氧化酶	+	脲酶	-	棉籽糖产酸	-
厌氧生长	-	吲哚产生	-	松二糖产酸	-
硝酸盐还原	+	ONPG	+	L-阿拉伯糖产酸	+
明胶液化	+	精氨酸双水解酶	-	D-葡萄糖产酸	+
VP	+	鸟氨酸脱羧酶	-	D-核糖产酸	+
淀粉水解	+	鸟氨酸脱羧酶	-	D-木糖产酸	+
七叶灵水解	+	乳糖产酸	+

生理生化试验	结果	生理生化试验	结果	生理生化试验	结果
接触酶	+	酪蛋白水解	+	蜜二糖产酸	-
氧化酶	+	脲酶	-	棉籽糖产酸	-
厌氧生长	-	吲哚产生	-	松二糖产酸	-
硝酸盐还原	+	ONPG	+	L-阿拉伯糖产酸	+
明胶液化	+	精氨酸双水解酶	-	D-葡萄糖产酸	+
VP	+	鸟氨酸脱羧酶	-	D-核糖产酸	+
淀粉水解	+	鸟氨酸脱羧酶	-	D-木糖产酸	+
七叶灵水解	+	乳糖产酸	+

病原真菌	抑菌带宽度/mm
烟草黑胫病菌Phytophthora parasitica var. nicotianae	0.00±0.00h
烟草赤星病菌Alternaria alternata	8.23±0.19c
烟草灰霉病菌Botrytis cinerea	6.27±0.17e
烟草炭疽病菌Colletotrichum nicotianae	7.63±0.37cd
烟草梨孢叶斑病菌Pyricularia oryzae	7.76±0.40cd
菠萝黑腐病菌Thielaviopsis paradoxa	11.80±0.33a
玉米腐霉茎腐病菌Pythium inflatum	0.00±0.00h
花生白绢病菌Sclerotium rolfsii	2.57±0.09g
花生立枯病菌Rhizoctonia solani	4.80±0.24f
水稻稻瘟病菌Magnaporthe oryzae	7.03±0.17d
水稻纹枯病菌Rhizoctonia solani	4.91±0.31f
剑兰弯孢叶斑病菌Curvularia gladioli	8.87±0.57bc
辣椒炭疽病菌Colletotrichum acutatum	7.57±0.29cd
香蕉枯萎病菌Fusarium oxysporum f.sp.cubense	9.03±0.46b
辣椒疫病菌Phytophthora capsici	0.00±0.00h