Antibacterial Activities of Litter Fungus Neocucurbitaria salicis-albae and Its Metabolite

doi:10.11924/j.issn.1000-6850.casb2023-0037

Abstract

Abstract:

This study focuses on discovering the antimicrobial fungal resources from forest litter fungi, screening out and identifying the fungal strains with antimicrobial activities, and isolating and purifying their antimicrobial compounds. The forest litter from the Greater Hinggan Mountains was selected as test materials, then the fungi were isolated by particle separation method, the taxonomic data were measured by gene sequence comparison, the antibacterial activity was measured by well diffusion method, the monomeric compounds were isolated by column chromatography, and the chemical structure was determined by spectral analysis. Fungal strain SGSF801 was isolated and identified as a new strain of Neocucurbitaria salicis-albae by multiple sequence analysis. The results of the antimicrobial assay showed that the rice fermentation products had strong antimicrobial activities against plant pathogens Ralstonia solanacearum, Pseudomonas syringae and Rhizoctonia solani. The product of fungal rice fermentation was isolated and purified to obtain a monomeric compound, which was identified as Cercosporamide by ¹H-NMR and ¹³C-NMR, and its molecular formula was C₁₆H₁₃NO₇. Its MIC values were 31.25-62.5 μg/mL for Ralstonia solanacearum and 125-250 μg/mL for Pseudomonas syringae. In this study, Cercosporamide was isolated from fungal genus Neocucurbitaria for the first time, and it was found to have antibacterial activities against plant pathogens, which provided a basis for further utilization of litter fungi resources.

Key words: fungal identification, antibacterial activity, Cercosporamide, secondary metabolite, Greater Hinggan Mountains

LIU Yao, ZHAO Wuchao, YANG Xiao, KOU Yuxin, DU Tingting, CHEN Xiuqing, QIN Mengfei, XU Lijian. Antibacterial Activities of Litter Fungus Neocucurbitaria salicis-albae and Its Metabolite[J]. Chinese Agricultural Science Bulletin, 2024, 40(9): 145-149.

Figures/Tables 5

References 22

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序列	最相近菌种	登录号	覆盖率/%	相似率/%
ITS	Neocucurbitaria salicis-albae	NR163365	100	99.10
LSU	Neocucurbitaria salicis-albae	MK442535	100	99.87
TUB2	Neocucurbitaria salicis-albae	MK442738	100	97.54

序列	最相近菌种	登录号	覆盖率/%	相似率/%
ITS	Neocucurbitaria salicis-albae	NR163365	100	99.10
LSU	Neocucurbitaria salicis-albae	MK442535	100	99.87
TUB2	Neocucurbitaria salicis-albae	MK442738	100	97.54

培养基	15℃	20℃	25℃	30℃
OA	16.00±1.63f	42.67±2.49a	41.3±1.89a	32.00±1.63bc
PDA	23.67±2.49de	29.67±1.25c	32.33±2.05bc	22.67±0.94de
YMA	20.33±2.62e	32.67±0.94bc	34.67±0.94b	30.67±0.94c
MEA	23.33±0.94de	34.67±0.94b	24.67±0.47d	24.33±0.47d

培养基	15℃	20℃	25℃	30℃
OA	16.00±1.63f	42.67±2.49a	41.3±1.89a	32.00±1.63bc
PDA	23.67±2.49de	29.67±1.25c	32.33±2.05bc	22.67±0.94de
YMA	20.33±2.62e	32.67±0.94bc	34.67±0.94b	30.67±0.94c
MEA	23.33±0.94de	34.67±0.94b	24.67±0.47d	24.33±0.47d

供试病原菌	PDA	PD	SM	大米
丁香假单胞菌	+	+	++	++
青枯劳尔氏菌	++	+	++	++
水稻黄单胞菌	—	—	—	—
玉米链格孢菌	—	—	—	—
立枯丝核菌	—	—	—	++