Four Food-derived Polysaccharides: Monosaccharide Composition and Their Inhibitory Effects on Methicillin-resistant Staphylococcus aureus

doi:10.11924/j.issn.1000-6850.casb2022-0712

Abstract

Abstract:

The research aims to provide a possible alternative to the problem of drug resistance in pathogenic bacteria. With fungus, yam, lily and wolfberry as raw materials, water extraction and alcohol precipitation method, ion chromatography, crystal violet staining and other techniques were used to compare and analyze the monosaccharide composition of four kinds of foodborne polysaccharides and their inhibitory effect on methicillin-resistant Staphylococcus aureus (MRSA). The results showed that glucose was the main monosaccharide of the four polysaccharides. Auricularia polysaccharides had significant inhibitory effects on the growth, biofilm formation, cell adhesion and toxicity of MRSA. Lilium polysaccharide and yam polysaccharide only had significant inhibitory effect on the cell adhesion and toxicity of MRSA, but had no significant inhibitory effect on the growth of MRSA and the formation of biofilm. Lycium barbarum polysaccharide had little inhibitory effect on MRSA. Auricularia auricularia polysaccharide has better inhibition ability against pathogenic bacteria MRSA than the other three foodborne polysaccharides. As a natural product with potential antibacterial applications against MRSA, it has the value of further characterization and optimization.

Key words: food-derived polysaccharide, monosaccharide composition, Methicillin-resistant Staphylococcus aureus, antibacterial activity

YIN Hong, ZHAO Shijing, XIAO Jiu, GAO Qifang, RAN Ganqiao, XU Dan. Four Food-derived Polysaccharides: Monosaccharide Composition and Their Inhibitory Effects on Methicillin-resistant Staphylococcus aureus[J]. Chinese Agricultural Science Bulletin, 2023, 39(24): 135-142.

Figures/Tables 8

References 38

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Sample ID	RT/min	lg Mp	lgMw	lgMn	Mp	Mw	Mn	峰面积比/%
1	27.574	6.6	6.8	6.5	3943765	6608185	3153456	64.569
2	39.059	4.6	4.7	4.5	40111	49981	33548	21.697
3	42.575	4.0	4.0	3.9	9845	11205	8349	13.734

Sample ID	RT/min	lg Mp	lgMw	lgMn	Mp	Mw	Mn	峰面积比/%
1	27.574	6.6	6.8	6.5	3943765	6608185	3153456	64.569
2	39.059	4.6	4.7	4.5	40111	49981	33548	21.697
3	42.575	4.0	4.0	3.9	9845	11205	8349	13.734

单糖类型	枸杞多糖	木耳多糖	山药多糖	百合多糖
半乳糖醛酸(Gal-UA)	6.10	0.33	2.81	2.47
阿拉伯糖(Ara)	13.18	0.17	0.75	0.99
半乳糖(Gal)	9.66	5.66	6.94	1.06
鼠李糖(Rha)	2.48	0.23	0.64	0.28
葡萄糖(Glc)	61.77	60.78	87.01	62.64
木糖(Xyl)	1.72	3.90	0.21	0.15
甘露糖(Man)	2.22	16.33	1.13	32.07
岩藻糖(Fuc)	0.19	2.87	0.07	0.08
葡萄糖醛酸(Glc-UA)	1.34	9.20	0.27	0.14
果糖(Fru)	1.04	-	-	-
核糖(Rib)	0.18	0.30	0.16	0.12
古罗糖醛酸(Gul-UA)	0.13	0.13	-	-

单糖类型	枸杞多糖	木耳多糖	山药多糖	百合多糖
半乳糖醛酸(Gal-UA)	6.10	0.33	2.81	2.47
阿拉伯糖(Ara)	13.18	0.17	0.75	0.99
半乳糖(Gal)	9.66	5.66	6.94	1.06
鼠李糖(Rha)	2.48	0.23	0.64	0.28
葡萄糖(Glc)	61.77	60.78	87.01	62.64
木糖(Xyl)	1.72	3.90	0.21	0.15
甘露糖(Man)	2.22	16.33	1.13	32.07
岩藻糖(Fuc)	0.19	2.87	0.07	0.08
葡萄糖醛酸(Glc-UA)	1.34	9.20	0.27	0.14
果糖(Fru)	1.04	-	-	-
核糖(Rib)	0.18	0.30	0.16	0.12
古罗糖醛酸(Gul-UA)	0.13	0.13	-	-