Overview of Glyphosate and Research Progress in Microbial Degradation

doi:10.11924/j.issn.1000-6850.casb2024-0200

Abstract

Abstract:

The herbicide glyphosate is widely used in agriculture because of its high efficiency and low cost, but it also poses certain harm. This paper provided a brief introduction to glyphosate toxicity and mechanism. It was clarified that the harm of glyphosate to water environment was mainly in the aspects of affecting water quality, aquatic organisms and ecosystems. The harm to soil was mainly reflected in the destruction of soil microbial ecology, the destruction of soil microbial community structure and function, and the influence of soil enzyme activity. The research progress of bioremediation of glyphosate-contaminated soil was introduced. The bioremediation of glyphosate-contaminated soil was mainly microbial degradation methods. Compared with non-biodegradation such as adsorption and photolysis, microbial degradation was more environmentally friendly, efficient and promising. Finally, the microbial degradation methods of glyphosate contaminated soil remediation were prospected, in order to provide reference for the application and improvement of glyphosate microbial degradation technology in glyphosate contaminated soil.

Key words: herbicide, glyphosate, contaminated soil, microbial degradation, soil remediation

GE Zhijian, YANG Fengshan, LI Kun, FU Haiyan, LIU Chunguang. Overview of Glyphosate and Research Progress in Microbial Degradation[J]. Chinese Agricultural Science Bulletin, 2024, 40(23): 68-74.

Figures/Tables 2

References 55

[1]	ZHANG W P, LI J Y, ZHANG Y M, et al. Characterization of a novel glyphosate-degrading bacterial species, Chryseobacterium sp. Y16C, and evaluation of its effects on microbial communities in glyphosate-contaminated soil[J]. Journal of hazardous materials, 2022, 432:128689.
[2]	POWLES S B. Evolved glyphosate-resistant weeds around the world: Lessons to be learnt[J]. Pest management science: Formerly pesticide science, 2008, 64(4):360-365.
[3]	CHEN Y, CHEN W J, HUANG Y, et al. Insights into the microbial degradation and resistance mechanisms of glyphosate[J]. Environmental research, 2022, 215:114153.
[4]	陈虎保, 朱惠香, 陈国海. 草甘膦的作用机理及部位[J]. 林业科技通讯, 1997(1):21-23.
[5]	CRUNP K. The potential effects of recall bias and selection bias on the epidemiological evidence for the carcinogenicity of glyphosate[J]. Risk analysis, 2020, 40(4):696-704. doi: 10.1111/risa.13440 pmid: 31889327
[6]	GUYTON K Z, LOOMIS D, GROSSE Y, et al. Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate[J]. The lancet oncology, 2015, 16(5):490-491.
[7]	PEILLEX C, PELLETIER M. The impact and toxicity of glyphosate and glyphosate-based herbicides on health and immunity[J]. Journal of immunotoxicology, 2020, 17(1):163-174. doi: 10.1080/1547691X.2020.1804492 pmid: 32897110
[8]	SINGH S, KUMAR V, GILL J P K, et al. Herbicide glyphosate: Toxicity and microbial degradation[J]. International journal of environmental research and public health, 2020, 17(20):7519.
[9]	WILLIAMS G M, KROES R, MUNRO I C. Safety evaluation and risk assessment of the herbicide roundup and its active ingredient, glyphosate, for humans[J]. Regulatory toxicology and pharmacology, 2000, 31(2):117-165.
[10]	TSUI M T, CHU L M. Aquatic toxicity of glyphosate-based formulations: Comparison between different organisms and the effects of environmental factors[J]. Chemosphere 2003, 52(7):1189-1197. doi: 10.1016/S0045-6535(03)00306-0 pmid: 12821000
[11]	CO E, CD N, LC A, et al. Acute toxicity of glyphosate based herbicide glycot on juvenile African cat fish clarias gariepinus (burchell 1822)[J]. Journal of fisheries & livestock production, 2017, 5(3):1-4.
[12]	ROY N M, CARNEIRO B, OCHS J. Glyphosate induces neurotoxicity in Zebrafish[J]. Environmental toxicology and pharmacology, 2016, 42:45-54. doi: 10.1016/j.etap.2016.01.003 pmid: 26773362
[13]	马晓洁. 草甘膦对大型溞生长繁殖及抗氧化系统的影响[D]. 新乡: 河南师范大学, 2019:10-42.
[14]	CAVALCANTE D G S M, MARTINEZ C B R, SOFIA S H. Genotoxic effects of roundup ® on the fish Prochiloduslineatus[J]. Mutation research/genetic toxicology and environmental mutagenesis, 2008, 655(1-2):41-46.
[15]	洪宇航. 草甘膦对中华绒螯蟹成蟹主要免疫指标的影响[J]. 江苏农业科学, 2018, 46(12):136-140.
[16]	廖艺钰, 惠吕佳, 严吉祥, 等. 草甘膦农药对斑马鱼的急性毒性和慢性毒性研究[J]. 广州化工, 2020, 48(21):66-68.
[17]	KREUTZ L C, BARCELLOS L J G, SILVA T O, et al. Acute toxicity test of agricultural pesticides on silver catfish (Rhamdiaquelen) fingerlings[J]. Ciênciarural, 2008, 38(4):1050-1055.
[18]	PERIRA H A, HERNANDES P R T, NETTO M S, et al. Adsorbents for glyphosate removal in contaminated waters: A review[J]. Environmental chemistry letters, 2021, 19(2):1525-1543.
[19]	SOARES D, SILVA L, DUARTE S, et al. Glyphosate use, toxicity and occurrence in food[J]. Foods, 2021, 10(11):2785.
[20]	STEMPVOORT D R V, ROY J W, BROWN S J, et al. Residues of the herbicide glyphosate in riparian groundwater in urban catchments[J]. Chemosphere, 2014, 95:455-463. doi: 10.1016/j.chemosphere.2013.09.095 pmid: 24206835
[21]	LYNDSAY S, REZA P. Glyphosate in runoff waters and in the root-zone: Areview[J]. Toxics, 2015, 3(4):462-480.
[22]	LI J, ZHANG W, LIN Z, et al. Emerging strategies for the bioremediation of the phenylurea herbicide diuron[J]. Frontiers in microbiology, 2021, 12:686509.
[23]	YU X M, YU T, YIN G H, et al. Glyphosate biodegradation and potential soil bioremediation by Bacillus subtilis strain Bs-15[J]. Genetics and molecular research, 2015, 14(4):14717-14730.
[24]	ERMAKOVA I T, KISELEVA N I, SHUSHKOVA T, et al. Bioremediation of glyphosate-contaminated soils[J]. Applied microbiology and biotechnology, 2010, 88:585-594. doi: 10.1007/s00253-010-2775-0 pmid: 20676632
[25]	PÉREZ RODRÍGUEZ M, MELO C, JIMÉNEZ E, et al. Glyphosate bioremediation through the sarcosine oxidase pathway mediated by Lysinibacillus sphaericus in soils cultivated with potatoes[J]. Agriculture, 2019, 9(10):217.
[26]	ACOSTA-CORTÉS A G, MARTINEZ-LEDEZMA C, LÓPEZ-CHUKEN U J, et al. Polyphosphate recovery by a native Bacillus cereus strain as a direct effect of glyphosate uptake[J]. The ISME journal, 2019, 13(6):1497-1505.
[27]	KRYUCHKOVA Y V, BURYGIN G L, GOGOLEVA N E, et al. Isolation and characterization of a glyphosate-degrading rhizosphere strain, Enterobacter cloacae K7[J]. Microbiological research, 2014, 169(1):99-105.
[28]	MASSOT F, GKOREZIS P, HAMME J V, et al. Isolation, biochemical and genomic characterization of glyphosate tolerant bacteria to perform microbe-assisted phytoremediation[J]. Frontiers in microbiology, 2021, 11:598507.
[29]	LERBS W, STOCK M, PARTHIER B. Physiological aspects of glyphosate degradation in Alcaligenes spec. strain GL[J]. Archives of microbiology, 1990, 153:146-150.
[30]	OBOJSKA A, TERNAN N G, LEJCZAK B, et al. Organophosphonate utilization by the Thermophile Geobacillus caldoxylosilyticus T20[J]. Applied and environmental microbiology, 2002, 68(4):2081-2084.
[31]	PIPKE R, AMRHEIN N, JACOB G S, et al. Metabolism of glyphosate in an Arthrobacter sp. GLP-1[J]. European journal of biochemistry, 1987, 165:267-273.
[32]	PEALOZA-VAZQUEZ A, MENA G L, HERRERA-ESTRELLA L, et al. Cloning and sequencing of the genes involved in glyphosate utilization by Pseudomonas pseudomallei [J]. Applied and environmental microbiology, 1995, 61(2):538-543.
[33]	BALTHAZOR T M, HALLAS L E. Glyphosate-degrading microorganisms from industrial activated sludge[J]. Applied and environmental microbiology, 1986, 51:432-434. doi: 10.1128/aem.51.2.432-434.1986 pmid: 16346999
[34]	FIRDOU S, IQBA S, ANWA S, et al. Identification and analysis of 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene from glyphosate-resistant Ochrobactrum intermedium Sq20[J]. Pest management science, 2018, 74(5):1184-1196.
[35]	MCAULIFE K S, HALLAL E, KULP C F. Glyphosate degradation by Agrobacterium radiobacter isolated from activated sludge[J]. Journal of industrial microbiology, 1990, 6:219-221.
[36]	JACOB G S, GARBO J R, HALLA L E, et al. Metabolism of glyphosate in Pseudomonas sp. strain LBr[J]. Applied and environmental microbiology, 1988, 54(12):2953-2958.
[37]	QUINN J P, PEDEN J M M, DICK R E. Carbon-phosphorus bond cleavage by gram-positive and gram-negative soil bacteria[J]. Applied microbiology and biotechnology, 1989, 31:283-287.
[38]	FAN J, YANG G, ZHAO H, et al. Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil[J]. Journal of general and applied microbiology, 2012, 58(4):263-271.
[39]	FU G, HEN Y, LI R, et al. Pathway and rate-limiting step of glyphosate degradation by Aspergillus oryzae A-F02[J]. Preparative biochemistry and biotechnology, 2017, 47(8):782-788.
[40]	KRZY’KO-LUPICK T, STROF W, KUB K, et al. The ability of soil-borne fungi to degrade organophosphonate carbon-to-phosphorus bonds[J]. Applied microbiology & biotechnology, 1997, 48(4):549-552.
[41]	KRZYŚKO-ŁUPICKA T, ORLIK A. The use of glyphosate as the sole source of phosphorus or carbon for the selection of soil-borne fungal strains capable to degrade this herbicide[J]. Chemosphere, 1997, 34(12):2601-2605.
[42]	BUJACZ B, WIECZOREK P, KRZYSKO-LUPICKA T, et al. Organophosphonate utilization by the wild-type strain of Penicillium notatum[J]. Applied and environmental microbiology, 1995, 61(8):2905-2910.
[43]	OBOJSKA A, LEJCZAK B, KUBRAK M. Degradation of phosphonates by Streptomycete isolates[J]. Applied microbiology and biotechnology, 1999, 51:872-876.
[44]	SVIRIDOV A V, SHUSHKOVA T V, ZELENKOVA N F, et al. Distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria Ochrobactrum anthropi and Achromobactersp[J]. Applied microbiology and biotechnology, 2012, 93:787-796.
[45]	CHEN Y, JIANG Z, WU D, et al. Development of a novel bio-organic fertilizer for the removal of atrazine in soil[J]. Journal of environmental management, 2019, 233:553-560. doi: S0301-4797(18)31508-1 pmid: 30597348
[46]	CONTRERAS M B C, CAMACHO J V, FERNANDEZ-MORALES F J, et al. Biodegradability improvement and toxicity reduction of soil washing effluents polluted with atrazine by means of electrochemical pre-treatment: Influence of the anode material[J]. Journal of environmental management, 2020, 255:109895.
[47]	MORILLO E, VILLAVERDE J. Advanced technologies for the remediation of pesticide-contaminated soils[J]. Science of the total environment, 2017, 586:576-597.
[48]	OUYANG W, HAO X, TYSKLIND M, et al. Typical pesticides diffuse loading and degradation pattern differences under the impacts of climate and land-use variations[J]. Environment international, 2020, 139:105717.
[49]	QU M, LI H, LI N, et al. Distribution of atrazine and its phytoremediation by submerged macrophytes in lake sediments[J]. Chemosphere, 2017, 168:1515-1522. doi: S0045-6535(16)31710-6 pmid: 27932038
[50]	WANG A, HU X, WAN Y, et al. A nationwide study of the occurrence and distribution of atrazine and its degradates in tap water and groundwater in China: Assessment of human exposure potential[J]. Chemosphere, 2020, 252:126533.
[51]	SVIRIDOV A V, SHUSHKOVA T V, ERMAKOVA I T, et al. Microbial degradation of glyphosate herbicides[J]. Applied biochemistry and microbiology, 2015, 51:188-195.
[52]	BAZOT S, LEBEAU T. Simultaneous mineralization of glyphosate and diuron by a consortium of three bacteria as free-and/or immobilized-cells formulations[J]. Applied microbiology and biotechnology, 2008, 77:1351-1358.
[53]	SHUSHKOVA T, ERMAKOVA I, LEONTIEVSKY A. Glyphosate bioavailability in soil[J]. Biodegradation, 2010, 21:403-410. doi: 10.1007/s10532-009-9310-y pmid: 19902365
[54]	胡浩. 生态修复技术在水环境保护中的应用[J]. 科技资讯, 2023, 21(14):108-111.
[55]	GÓNGORA-ECHEVERRÍA V R, GARCÍA-ESCALANTE R, ROJAS-HERRERA R, et al. Pesticide bioremediation in liquid media using a microbial consortium and bacteria-pure strains isolated from a biomixture used in agricultural areas[J]. Ecotoxicology and environmental safety, 2020, 200:110734.

微生物种类	拉丁文名称	中文名称	参考文献
细菌	Arthrobacter sp. GLP-1	节杆菌GLP-1	[31]
	Alcaligenes sp. GL	产碱菌GL	[29]
	Pseudomonas pseudomallei22	类鼻疽假单胞菌22	[32]
	Flavobacterium sp. GD1	黄杆菌GD1	[33]
	Ochrobactrum intermedium Sq20	中间苍白杆菌Sq20	[34]
	Agrobacterium radiobacterSW9	放射杆状土壤杆菌SW9	[35]
	Achromobacter sp. LW9	无色杆菌LW9	[35]
	Pseudomonas sp. LBr	假单胞菌LBr	[36]
	Pseudomonas sp. 4ASW	假单胞菌4ASW	[37]
	Bacillus megaterium 2BLW	巨大芽胞杆菌2BLW	[37]
	Bacillus cereus CB4	蜡样芽孢杆菌CB4	[38]
真菌	Aspergillus oryzae A-F02	米曲霉A-F02	[39]
	Aspergillus niger	黑曲霉	[40]
	Penicillium IIR	青霉菌IIR	[41]
	Mucor IIIR	毛霉菌III	[41]
	Scopulariopsis sp.	帚霉菌	[40]
	Trichoderma harzianum	哈茨木霉	[40]
	Penicillium notatum	点青霉	[42]
放线菌	Streptomycete isolates	链霉菌	[43]

微生物种类	拉丁文名称	中文名称	参考文献
细菌	Arthrobacter sp. GLP-1	节杆菌GLP-1	[31]
	Alcaligenes sp. GL	产碱菌GL	[29]
	Pseudomonas pseudomallei22	类鼻疽假单胞菌22	[32]
	Flavobacterium sp. GD1	黄杆菌GD1	[33]
	Ochrobactrum intermedium Sq20	中间苍白杆菌Sq20	[34]
	Agrobacterium radiobacterSW9	放射杆状土壤杆菌SW9	[35]
	Achromobacter sp. LW9	无色杆菌LW9	[35]
	Pseudomonas sp. LBr	假单胞菌LBr	[36]
	Pseudomonas sp. 4ASW	假单胞菌4ASW	[37]
	Bacillus megaterium 2BLW	巨大芽胞杆菌2BLW	[37]
	Bacillus cereus CB4	蜡样芽孢杆菌CB4	[38]
真菌	Aspergillus oryzae A-F02	米曲霉A-F02	[39]
	Aspergillus niger	黑曲霉	[40]
	Penicillium IIR	青霉菌IIR	[41]
	Mucor IIIR	毛霉菌III	[41]
	Scopulariopsis sp.	帚霉菌	[40]
	Trichoderma harzianum	哈茨木霉	[40]
	Penicillium notatum	点青霉	[42]
放线菌	Streptomycete isolates	链霉菌	[43]