Abstract: The tobacco leaves were cured after spraying with each of the ten isolates and then were examined for their chemical components and the inner quality change. The results indicated that the chemical substances and the smoking quality of the treated leaves were affected in some extent and the different position leaves was affected by all the bacteria strains significantly. The middle-position leaves were treated by the isolates 1~6# isolate, the nicotine reduction rate of tobacco leaves sprayed with 4# was 36.9%, which is the highest of all tested isolates. The super-position leaves were treated by 1~11# isolates, the nicotine reduction rate with 9# was 37.25% and the next rate was the treatment of 10# and 4# isolate, 22.80% and 19.86% respectively. The leaves treated by 4#, 9#, 10# were determined their inner quality by smoking and the results showed that the total scores of 4# and 9# treatment was higher than the control. The smoking quality of treatment applied with that increased in terms of the aroma quality and volume, and the irritation strength was reduced.