Analysis of Genetic Diversity and Genetic Differentiation of Six Geographical Populations of Bellamyapurificata

doi:10.11924/j.issn.1000-6850.casb2024-0165

Abstract

Abstract:

The study aims to understand the genetic characteristics of Bellamya purificata populations in China. In this paper, six populations of B. purificata were selected from Wuhu of Anhui (ASY), Huai 'an of Jiangsu (CX), Nanhai of Guangxi (GZT), Jiangyin of Jiangsu (JY), Changzhou of Jiangsu (RS) and Liuzhou of Guangxi (ZZR), and their genetic diversity, population structure and selection signals were compared. The results showed that the genetic diversity of these six populations was high, and the single nucleotide polymorphism (π) was 0.005976-0.007760. Among them, the genetic diversity of GZT population and ASY population was relatively higher, while the genetic diversity of ZZR population was lower. There was a large separation between RS population and GZT population, and the separation between ZZR population and ASY population was small. The separation between the remaining populations was not obvious. It shows that although the population of B. purificata is widely distributed, the genetic differentiation between different geographical populations is not large.

Key words: Bellamya purificata, population structure, population genetics, genetic diversity, SNP molecular marker, genetic breeding, genetic differentiation, single nucleotide polymorphisms, divergence

LI Ke, MA Xueyan, YANG Ke, CHEN Wanwen, JIN Wu, WEN Haibo. Analysis of Genetic Diversity and Genetic Differentiation of Six Geographical Populations of Bellamyapurificata[J]. Chinese Agricultural Science Bulletin, 2024, 40(29): 150-156.

Figures/Tables 10

References 29

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群体	时间（年-月-日）	地点	养殖模式
安徽芜湖(ASY)	2021-09-12	31.271005°N, 118.356932°E	池塘散养
江苏淮安(CX)	2021-06-30	33.110026°N,118.74366°E	稻螺共作
广西南海(GZT)	2021-08-13	25.610574°N,109.623329°E	稻螺共作
江苏江阴(JY)	2021-03-15	31.796743°N,120.196434°E	鱼苗夏花池套养
江苏常州(RS)	2021-06-09	31.766999°N,120.188828°E	家鱼夏花池套养
广西柳州(ZZR)	2021-08-03	24.363546°N,109.278187°E	稻螺共作

群体	时间（年-月-日）	地点	养殖模式
安徽芜湖(ASY)	2021-09-12	31.271005°N, 118.356932°E	池塘散养
江苏淮安(CX)	2021-06-30	33.110026°N,118.74366°E	稻螺共作
广西南海(GZT)	2021-08-13	25.610574°N,109.623329°E	稻螺共作
江苏江阴(JY)	2021-03-15	31.796743°N,120.196434°E	鱼苗夏花池套养
江苏常州(RS)	2021-06-09	31.766999°N,120.188828°E	家鱼夏花池套养
广西柳州(ZZR)	2021-08-03	24.363546°N,109.278187°E	稻螺共作

调查点	数量/个	平均壳长/mm	平均体重/g	平均壳高/mm	年龄/year	水温/℃
安徽芜湖(ASY)	30	29.96±0.55	5.01±0.21	21.10±0.36	2	25
江苏淮安(CX)	30	26.41±0.35	0.71±0.13	16.15±0.16	2	26.4
广西南海(GZT)	30	29.11±0.42	0.92±0.17	17.57±0.34	2	20.8
江苏江阴(JY)	30	34.13±0.47	1.01±0.18	20.03±0.23	3	24.5
江苏常州(RS)	30	28.53±0.59	0.73±0.13	17.73±0.33	2	28.2
广西柳州(ZZR)	30	23.85±0.49	0.91±0.17	14.83±0.32	2	20.8

调查点	数量/个	平均壳长/mm	平均体重/g	平均壳高/mm	年龄/year	水温/℃
安徽芜湖(ASY)	30	29.96±0.55	5.01±0.21	21.10±0.36	2	25
江苏淮安(CX)	30	26.41±0.35	0.71±0.13	16.15±0.16	2	26.4
广西南海(GZT)	30	29.11±0.42	0.92±0.17	17.57±0.34	2	20.8
江苏江阴(JY)	30	34.13±0.47	1.01±0.18	20.03±0.23	3	24.5
江苏常州(RS)	30	28.53±0.59	0.73±0.13	17.73±0.33	2	28.2
广西柳州(ZZR)	30	23.85±0.49	0.91±0.17	14.83±0.32	2	20.8

群体	单核苷酸密度(SNP/Kb)	单核苷酸多态性(π)	多态性信息含量(PIC)	观测杂合度(Ho)	近交系数(F_HOM)
ASY	38.10	0.006764	0.156±0.115	0.131±0.123	0.273
CX	43.19	0.007760	0.151±0.116	0.135±0.126	0.221
GZT	37.89	0.006976	0.155±0.116	0.149±0.145	0.168
JY	43.23	0.007360	0.146±0.119	0.128±0.124	0.231
RS	32.08	0.005976	0.158±0.119	0.163±0.161	0.104
ZZR	42.56	0.007203	0.148±0.112	0.130±0.127	0.246
总群体	73.66	0.007681	0.091±0.111	0.069±0.096	0.282