The Synergistic Antioxidant Activity of Three Berry Anthocyanins: Study Based on Uniform Design in Vitro

doi:10.11924/j.issn.1000-6850.casb2024-0393

Abstract

Abstract:

In order to study the synergistic antioxidant activity of three types of berry anthocyanins, the DPPH free radical scavenging rate was used as an indicator. The uniform experimental design method was used to optimize the synergistic antioxidant combination of three types of berry anthocyanins. After statistical analysis and mathematical regression model fitting, the optimal combination was obtained: Vaccinium uliginosum L. anthocyanin (DP), Ribes nigrum L. anthocyanin (HP), and Lonicera caerulea L. var. edulis Turcz. ex Herd. anthocyanin (LP) with optimal concentrations of 0.08 mg/mL, 0.14 mg/mL, and 0.18 mg/mL, respectively. The actual scavenging rate of DPPH free radicals was (97.67±0.51)%, which was close to the theoretical expected value of 100%, indicating the feasibility of the optimized combination. Through synergistic effect analysis, the CI values of the synergistic effect index for clearing DPPH free radicals and hydroxyl free radicals in the optimal combination were 0.297 and 0.651, respectively, further indicating that the purified anthocyanins of DP, HP, and LP have significant synergistic antioxidant effects. The research results can provide a basis for the development of natural antioxidant products and small berry functional products.

Key words: Vaccinium uliginosum L., Ribes nigrum L., Lonicera caerulea L. var. edulis Turcz.ex Herd., anthocyanins, uniform design, synergistic effect analysis, antioxidant, synergistic antioxidant activity, uniform test design method, DPPH free radical scavenging rate

WANG Hua, JIN Huanhuan, YU Xiaoying, LI Mengsha, HE Danrao, ZHOU Liping. The Synergistic Antioxidant Activity of Three Berry Anthocyanins: Study Based on Uniform Design in Vitro[J]. Chinese Agricultural Science Bulletin, 2024, 40(30): 141-148.

Figures/Tables 7

References 28

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试验运行号	编码变量			过程变量/(mg/mL)			实测值	目标值
试验运行号	X₁	X₂	X₃	X₁(DP)	X₂(HP)	X₃(LP)	清除率Y/%	清除率Y/%
1	1	5	13	0.0000	0.0345	0.1030	66.66±3.01	64.81
2	2	10	10	0.0085	0.0770	0.0770	70.07±1.64	66.84
3	3	15	7	0.0170	0.1200	0.0515	69.62±3.14	69.02
4	4	4	4	0.0255	0.0255	0.0255	50.28±2.85	51.52
5	5	9	1	0.0345	0.0685	0.0000	54.22±1.76	53.75
6	6	14	14	0.0430	0.1115	0.1115	75.76±2.64	80.77
7	7	3	11	0.0515	0.0170	0.0855	62.58±2.56	63.27
8	8	8	8	0.0600	0.0600	0.0600	69.39±1.18	65.46
9	9	13	5	0.0685	0.1030	0.0345	66.39±3.62	67.64
10	10	2	2	0.077	0.0085	0.0085	46.169±2.64	50.14
11	11	7	15	0.0855	0.0515	0.1200	77.99±3.17	77.16
12	12	12	12	0.0945	0.0945	0.0945	79.35±0.19	79.39
13	13	1	9	0.1030	0.0000	0.0685	62.80±2.32	61.89
14	14	6	6	0.1115	0.0430	0.0430	66.75±1.81	64.07
15	15	11	3	0.1200	0.0855	0.0170	69.18±1.67	66.09
推测值	8	14	18	0.0790	0.1406	0.1788	97.67±0.51	100.00

试验运行号	编码变量			过程变量/(mg/mL)			实测值	目标值
试验运行号	X₁	X₂	X₃	X₁(DP)	X₂(HP)	X₃(LP)	清除率Y/%	清除率Y/%
1	1	5	13	0.0000	0.0345	0.1030	66.66±3.01	64.81
2	2	10	10	0.0085	0.0770	0.0770	70.07±1.64	66.84
3	3	15	7	0.0170	0.1200	0.0515	69.62±3.14	69.02
4	4	4	4	0.0255	0.0255	0.0255	50.28±2.85	51.52
5	5	9	1	0.0345	0.0685	0.0000	54.22±1.76	53.75
6	6	14	14	0.0430	0.1115	0.1115	75.76±2.64	80.77
7	7	3	11	0.0515	0.0170	0.0855	62.58±2.56	63.27
8	8	8	8	0.0600	0.0600	0.0600	69.39±1.18	65.46
9	9	13	5	0.0685	0.1030	0.0345	66.39±3.62	67.64
10	10	2	2	0.077	0.0085	0.0085	46.169±2.64	50.14
11	11	7	15	0.0855	0.0515	0.1200	77.99±3.17	77.16
12	12	12	12	0.0945	0.0945	0.0945	79.35±0.19	79.39
13	13	1	9	0.1030	0.0000	0.0685	62.80±2.32	61.89
14	14	6	6	0.1115	0.0430	0.0430	66.75±1.81	64.07
15	15	11	3	0.1200	0.0855	0.0170	69.18±1.67	66.09
推测值	8	14	18	0.0790	0.1406	0.1788	97.67±0.51	100.00

模型	平方和	自由度	均方	F	P	显著性
回归	1180.465	3	393.488	48.104	0.000	^**
残差	89.979	11	8.180
总计	1270.444	14
R²=0.929			R_adj²=0.910

模型	平方和	自由度	均方	F	P	显著性
回归	1180.465	3	393.488	48.104	0.000	^**
残差	89.979	11	8.180
总计	1270.444	14
R²=0.929			R_adj²=0.910

模型	未标准化系数		标准化系数	t	P	显著性
模型	B	Sd	标准化系数	t	P	显著性
(常量)	41.223	2.351		17.534	0.000	^**
DP	86.040	20.303	0.347	4.238	0.001	^**
HP	142.532	20.178	0.574	7.064	0.000	^**
LP	181.299	20.178	0.730	8.985	0.000	^**