Explanation of Integrative Analysis of Metabolome and Transcriptome for Anthocyanins Synthesis Mechanism in Cassava Leaves

doi:10.11924/j.issn.1000-6850.casb2023-0432

Abstract

Abstract:

Cassava is the sixth largest staple food crop in the world, which storage root is rich in starch while lack of proteins, anthocyanins and carotenes and so on. Two types of leaves of cassava with different color (FL and PL) were selected as materials to explore the mechanism of anthocyanin biosynthesis by combining with transcriptome and metabolome in this work. The transcriptome results showed that there were 6864 differentially expressed genes (DEGs) including 4112 up-regulating and 2752 down-regulating in PL compare with FL. The metabolome results showed that there were 26 significantly different metabolites (SDMs) in PL compare with FL, and 21 of them belonged to anthocyanins. The result of combination of transcriptome and metabolome showed that there were seven DEGs related to anthocyanin biosynthesis and all of them were positively correlated to anthocyanin biosynthesis. The expression of MeANS1 showed the most difference. It provided candidate genes for elucidating the mechanism of anthocyanin biosynthesis in cassava and laid a scientific foundation for enhancement content of anthocyanin in future.

Key words: cassava, flavonoid, anthocyanins, transcriptome, metabolome, biosynthesis, differentially expressed genes, MeANS

LUO Xiuqin, WEI Zhuowen, CAI Jie, AN Feifei, CHEN Songbi, XUE Jingjing. Explanation of Integrative Analysis of Metabolome and Transcriptome for Anthocyanins Synthesis Mechanism in Cassava Leaves[J]. Chinese Agricultural Science Bulletin, 2024, 40(24): 100-106.

Figures/Tables 7

References 16

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样品	Raw Reads	Clean Reads	Clean Base(G)	错误率/%	Q20/%	Q30/%	GC含量t/%
FL	49851289.33	48392253	7.26	0.03	97.50667	92.95333	43.75333
PL	48613812.67	47113263	7.066667	0.03	97.42333	92.80667	43.31

样品	Raw Reads	Clean Reads	Clean Base(G)	错误率/%	Q20/%	Q30/%	GC含量t/%
FL	49851289.33	48392253	7.26	0.03	97.50667	92.95333	43.75333
PL	48613812.67	47113263	7.066667	0.03	97.42333	92.80667	43.31

物质	物质类别	FL/(ng/g)	PL/(ng/g)	倍数(FL&PL)
矢车菊素-3-(6-O-p-咖啡酰)-葡萄糖苷	矢车菊素	2.7196±0.7705B	26.2989±0.7705A	9.6701
矢车菊素-3,5-O-二葡萄糖苷		0.0828±0.0021B	2.6097±0.0685A	31.5192
矢车菊素-3-O-葡萄糖苷		0.4664±0.0188B	11.9382±0.1446A	25.5939
矢车菊素-3-O-芸香糖苷		1.7501±0.0720B	24.9373±0.4226A	14.2494
矢车菊素-3-O-桑布双糖苷		0.0160±0.0013B	0.4867±0.0411A	30.3952
矢车菊素-3-O-槐糖苷		0.0445±0.0017B	1.8697±0.0323A	41.9972
矢车菊素-3-O-木糖苷		0.0115±0.0008B	0.1795±0.0030A	15.5381
飞燕草素	飞燕草素	0.17204±0.0034B	1.2881±0.0175A	7.4873
飞燕草素-3,5-O-二葡萄糖苷		0.1259±0.0028B	8.7282±0.2205A	69.3402
飞燕草素-3-O-葡萄糖苷		1.0915±0.0287B	9.3198±0.3368A	8.5386
飞燕草素-3-O-鼠李糖苷		0.06500±0.0009B	0.3599±0.0473A	5.5384
飞燕草素-3-O-芸香糖苷		5.0056±0.1091B	295.9350±6.2067A	59.1209
飞燕草素-3-O-芸香糖苷-5-O-葡萄糖苷		0.1837±0.0122B	0.5204±0.0295A	2.8338
锦葵色素-3-O-半乳糖苷	锦葵色素	0.0011±0.0001B	0.0107±0.0009A	9.8393
锦葵色素-3-O-葡萄糖苷	锦葵色素	0.0119±0.0003B	0.05289±0.0011A	4.4336
天竺葵素-3-O-葡萄糖苷	天竺葵素	0.0057±0.0004B	0.0764±0.0023A	13.4108
天竺葵素-3-O-芸香糖苷	天竺葵素	0.3995±0.00259B	15.8928±0.3032A	39.7795
芍药花素-3-O-葡萄糖苷	芍药花素	0.0012±0.0001B	0.1918±0.0058A	166.2545
芍药花素-3-O-芸香糖苷	芍药花素	0.1620±0.0045B	9.6148±0.2802A	59.3364
矮牵牛素-3-O-葡萄糖苷	矮牵牛素	0.0380±0.0006B	0.2716±0.0106A	7.1428
矮牵牛素-3-O-芸香糖苷	矮牵牛素	0.1446±0.0025B	3.6052±0.0914A	24.9263
总花青素		12.4987±0.0474B	414.1877±6.9967A	33.1385

物质	物质类别	FL/(ng/g)	PL/(ng/g)	倍数(FL&PL)
矢车菊素-3-(6-O-p-咖啡酰)-葡萄糖苷	矢车菊素	2.7196±0.7705B	26.2989±0.7705A	9.6701
矢车菊素-3,5-O-二葡萄糖苷		0.0828±0.0021B	2.6097±0.0685A	31.5192
矢车菊素-3-O-葡萄糖苷		0.4664±0.0188B	11.9382±0.1446A	25.5939
矢车菊素-3-O-芸香糖苷		1.7501±0.0720B	24.9373±0.4226A	14.2494
矢车菊素-3-O-桑布双糖苷		0.0160±0.0013B	0.4867±0.0411A	30.3952
矢车菊素-3-O-槐糖苷		0.0445±0.0017B	1.8697±0.0323A	41.9972
矢车菊素-3-O-木糖苷		0.0115±0.0008B	0.1795±0.0030A	15.5381
飞燕草素	飞燕草素	0.17204±0.0034B	1.2881±0.0175A	7.4873
飞燕草素-3,5-O-二葡萄糖苷		0.1259±0.0028B	8.7282±0.2205A	69.3402
飞燕草素-3-O-葡萄糖苷		1.0915±0.0287B	9.3198±0.3368A	8.5386
飞燕草素-3-O-鼠李糖苷		0.06500±0.0009B	0.3599±0.0473A	5.5384
飞燕草素-3-O-芸香糖苷		5.0056±0.1091B	295.9350±6.2067A	59.1209
飞燕草素-3-O-芸香糖苷-5-O-葡萄糖苷		0.1837±0.0122B	0.5204±0.0295A	2.8338
锦葵色素-3-O-半乳糖苷	锦葵色素	0.0011±0.0001B	0.0107±0.0009A	9.8393
锦葵色素-3-O-葡萄糖苷	锦葵色素	0.0119±0.0003B	0.05289±0.0011A	4.4336
天竺葵素-3-O-葡萄糖苷	天竺葵素	0.0057±0.0004B	0.0764±0.0023A	13.4108
天竺葵素-3-O-芸香糖苷	天竺葵素	0.3995±0.00259B	15.8928±0.3032A	39.7795
芍药花素-3-O-葡萄糖苷	芍药花素	0.0012±0.0001B	0.1918±0.0058A	166.2545
芍药花素-3-O-芸香糖苷	芍药花素	0.1620±0.0045B	9.6148±0.2802A	59.3364
矮牵牛素-3-O-葡萄糖苷	矮牵牛素	0.0380±0.0006B	0.2716±0.0106A	7.1428
矮牵牛素-3-O-芸香糖苷	矮牵牛素	0.1446±0.0025B	3.6052±0.0914A	24.9263
总花青素		12.4987±0.0474B	414.1877±6.9967A	33.1385