Acceleration of Genetic Improvement of Vegetable Crops by CRISPR/Cas Gene Editing System

doi:10.11924/j.issn.1000-6850.casb2023-0722

Abstract

Abstract:

Modern breeding techniques based on biotechnology have a great impact on crop genetic improvement. CRISPR/Cas gene editing system’s components, mechanisms, breakthrough progresses of gene editing technology in the gene functional verification and crop genetic improvement, current utilization in various vegetable crops such as tomato, watermelon, cabbage, carrot, cucumber, and others, were summarized in this review. The CRISPR/Cas gene editing system stands out as the most extensively utilized gene-editing tool. This review aims to enhance comprehension of the CRISPR/Cas gene editing system and promote its vital role in vegetable crop improvement. This review discusses the factors affecting genetic transformation efficiency, methods to improve genetic transformation efficiency, the defects of the CRISPR gene editing system and the constraints in its current utilization in vegetable crops, including challenges related to the transformation and regeneration processes, and the genotype dependent for certain crops. In conclusion, this review suggests that future research should focus on extensive genotype screening to facilitate easier genetic transformation, the advancement of efficient crop transformation and regeneration systems, and the development of more efficient, innovative, precise, and multi-functional gene editing tools.

Key words: modern breeding techniques, CRISPR/Cas system, CRISPR gene editing, gene editing efficiency, gene functional verification, crop genetic improvement, vegetable crop improvement, genetic transformation efficiency, biotechnology

REN Wenjing, SI Jinchao, CHEN Li, YANG Limei, ZHUANG Mu, LV Honghao, WANG Yong, JI Jialei, ZHANG Yangyong. Acceleration of Genetic Improvement of Vegetable Crops by CRISPR/Cas Gene Editing System[J]. Chinese Agricultural Science Bulletin, 2024, 40(24): 107-115.

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