Effects of Salt Stress on Growth and Tissue Enzyme Activity of Juvenile Channel Catfish (Ictalurus punctatus)

doi:10.11924/j.issn.1000-6850.casb2024-0481

Abstract

Abstract:

To investigate the effects of salinity stress on the growth and antioxidant capacity of channel catfish fry, we established six salinity groups (3.0, 3.8, 4.7, 5.9, 7.3, 9.0) and one control group using the equidistant spacing method, the tolerance of each group to different salinity levels was assessed by measuring growth indexes, four physicochemical indexes, and H&E stained sections. The findings demonstrated that the growth performance and survival rates of fish fry at salinities of 3.0, 3.8, and 4.7 did not differ significantly (P>0.05). The growth rate and survival rate of fish fry gradually slowed down with the increase of salinity. The survival rate fell to 71% when the salinity hit 9.0. As salinity increased, the activity of three antioxidant enzymes—superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX)—in liver tissue first increased and then decreased. SOD and CAT activities were considerably increased (P<0.05) at a salinity of 4.7, with values of 1163.918 U/mg prot and 88.039 U/mg prot, respectively. The Hematoxylin and Eosin (H&E) stained sections results revealed significant alterations in a variety of tissues under varying salinity settings. These findings suggest that both low and high salt stress can cause damage to fish bodies, with liver tissue being particularly sensitive to salt stress. This research provides a theoretical foundation and essential data for the scientific cultivation of channel catfish fry in coastal mudflats.

Key words: channel catfish, growth performance, salinity stress, antioxidant enzyme, physiological index, H&E stained sections

DUAN Yongqiang, LIU Hongyan, WANG Minghua, ZHONG Liqiang, SU Chaofan, LIU Ju, CHEN Xiaohui, ZHANG Shiyong. Effects of Salt Stress on Growth and Tissue Enzyme Activity of Juvenile Channel Catfish (Ictalurus punctatus)[J]. Chinese Agricultural Science Bulletin, 2025, 41(5): 143-150.

Figures/Tables 3

References 47

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盐度	体长/c	体重/g	肝体比/%	脏体比/%	肥满度/(g/cm3)	特定生长率/(%/d)	存活率/%	饵料系数
0	11.162±0.605a	18.71±2.81a	0.789±0.080b	5.858±0.417a	1.341±0.097b	4.732±0.069a	0.927±0.009a	1.835±0.027c
3.0	10.786±0.580ab	17.21±2.69ab	0.780±0.144b	5.908±0.715a	1.363±0.107b	4.730±0.120a	0.900±0.016ab	1.869±0.133c
3.8	10.936±0.638ab	17.04±2.79ab	0.796±0.154b	5.516±0.580ab	1.303±0.143b	4.692±0.072a	0.907±0.034ab	1.893±0.072c
4.7	10.549±0.616bc	15.47±3.18bc	0.747±0.138b	5.247±0.442b	1.299±0.130b	4.494±0.063ab	0.853±0.025abc	2.112±0.087bc
5.9	10.210±0.798^c	14.84±4.00^c	0.831±0.158^b	5.735±0.674^ab	1.357±0.147^b	4.435±0.146^ab	0.750±0.090^cd	2.223±0.183^bc
7.3	9.944±0.703^c	13.27±3.05^c	0.878±0.242^b	5.700±0.545^ab	1.330±0.129^b	4.237±0.164^b	0.780±0.065^bcd	2.501±0.217^b
9.0	8.176±0.680^d	7.89±2.07^d	1.257±0.370^a	6.631±0.618^a	1.432±0.123^a	3.332±0.110^c	0.710±0.041^d	4.455±0.241^a

盐度	体长/c	体重/g	肝体比/%	脏体比/%	肥满度/(g/cm3)	特定生长率/(%/d)	存活率/%	饵料系数
0	11.162±0.605a	18.71±2.81a	0.789±0.080b	5.858±0.417a	1.341±0.097b	4.732±0.069a	0.927±0.009a	1.835±0.027c
3.0	10.786±0.580ab	17.21±2.69ab	0.780±0.144b	5.908±0.715a	1.363±0.107b	4.730±0.120a	0.900±0.016ab	1.869±0.133c
3.8	10.936±0.638ab	17.04±2.79ab	0.796±0.154b	5.516±0.580ab	1.303±0.143b	4.692±0.072a	0.907±0.034ab	1.893±0.072c
4.7	10.549±0.616bc	15.47±3.18bc	0.747±0.138b	5.247±0.442b	1.299±0.130b	4.494±0.063ab	0.853±0.025abc	2.112±0.087bc
5.9	10.210±0.798^c	14.84±4.00^c	0.831±0.158^b	5.735±0.674^ab	1.357±0.147^b	4.435±0.146^ab	0.750±0.090^cd	2.223±0.183^bc
7.3	9.944±0.703^c	13.27±3.05^c	0.878±0.242^b	5.700±0.545^ab	1.330±0.129^b	4.237±0.164^b	0.780±0.065^bcd	2.501±0.217^b
9.0	8.176±0.680^d	7.89±2.07^d	1.257±0.370^a	6.631±0.618^a	1.432±0.123^a	3.332±0.110^c	0.710±0.041^d	4.455±0.241^a