Biological Effects of Trichoderma W1 Bio-organic Fertilizer on Coastal Tomato Planting

doi:10.11924/j.issn.1000-6850.casb2024-0479

Abstract

Abstract:

In order to investigate the impact of Trichoderma-enriched bio-organic fertilizer on the yield, quality and soil nutrient availability in tomatoes cultivated in costal saline soil, the salt tolerance and mycelial morphology of two Trichoderma spp. were examined using plate growth. A field experiment was conducted in Ningbo coastal saline soil, with five treatment groups: no fertilizer (CK), chemical fertilizer at a rate of 2200 kg/hm² (T₁), bio-organic fertilizer at a rate of 9 t/hm² (T₂), bio-organic fertilizer at a rate of 18 t/hm² (T₃), and bio-organic fertilizer combined with chemical fertilizer at rates of 6 t/hm²+1500 kg/hm²(T₄), respectively. The bio-organic fertilizer was prepared using T. asperellum W1 strain. Lab experiment indicated that T. asperellum W1 exhibited superior growth ability compared to Trichoderma LX-132, thus validating its suitability for further testing. Based on phylogenetic tree analysis and mycelial morphology, W1 was provisionally identified as T. asperellum. Field experiment demonstrated that the application of bio-organic fertilizer resulted in higher organic matter content compared to non-bio-organic fertilizer treatments. Specifically, T₃ showed an increase in organic matter content by 16.5% and 10.7% when compared with CK and T₁, respectively. The bio-organic fertilizer treatment also led to improved quality and higher yields, with T₃ having the highest Vc content and sugar-acid ratio. These values increased by 5.39% and 6.18% compared to CK, and by 2.35% and 4.71% compared to T₁, respectively. The combination of bio-organic and chemical fertilizer (T₄) exhibited the most significant yield-increasing effect, with T₄ showing an increase of 117% and 6.26% compared to CK and T₁, respectively. The results of this research provide technical support and a theoretical foundation for the development of new bio-organic fertilizers.

Key words: saline land, bio-organic fertilizer, Trichoderma, Trichoderma asperellum, salt tolerance, soil nutrient, tomato, quality

WANG Lili, ZHU Shijun, LU Xiaohong, JIN Shuquan. Biological Effects of Trichoderma W1 Bio-organic Fertilizer on Coastal Tomato Planting[J]. Chinese Agricultural Science Bulletin, 2025, 41(7): 100-106.

Figures/Tables 6

References 37

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处理	pH	有机质/(g/kg)	全氮/(g/kg)	碱解氮/(mg/kg)	速效磷/(mg/kg)	速效钾/(mg/kg)
CK	7.48±0.06a	10.10±0.30c	1.05±0.06c	61.13±3.75c	48.37±4.70b	82.00±7.14c
T₁	7.46±0.06a	10.63±0.70bc	1.72±0.03a	89.00±1.97a	80.87±5.91a	196.27±6.39a
T₂	7.34±0.05b	11.53±0.55ab	1.60±0.03b	83.80±3.15b	73.80±1.37a	169.93±5.26b
T₃	7.24±0.08c	11.77±0.49a	1.59±0.06b	84.77±2.45b	75.77±3.06a	172.77±3.40b
T₄	7.36±0.10b	11.37±0.47ab	1.74±0.03a	87.30±2.61ab	75.43±1.82a	189.23±1.80a

处理	pH	有机质/(g/kg)	全氮/(g/kg)	碱解氮/(mg/kg)	速效磷/(mg/kg)	速效钾/(mg/kg)
CK	7.48±0.06a	10.10±0.30c	1.05±0.06c	61.13±3.75c	48.37±4.70b	82.00±7.14c
T₁	7.46±0.06a	10.63±0.70bc	1.72±0.03a	89.00±1.97a	80.87±5.91a	196.27±6.39a
T₂	7.34±0.05b	11.53±0.55ab	1.60±0.03b	83.80±3.15b	73.80±1.37a	169.93±5.26b
T₃	7.24±0.08c	11.77±0.49a	1.59±0.06b	84.77±2.45b	75.77±3.06a	172.77±3.40b
T₄	7.36±0.10b	11.37±0.47ab	1.74±0.03a	87.30±2.61ab	75.43±1.82a	189.23±1.80a

处理	可溶性固形物/%	可滴定酸/%	维生素C/(µg/mL)	糖酸比	产量/(kg/hm²)
CK	5.00±0.10b	1.40±0.02a	85.40±1.01c	3.56±0.10c	33939 ±2829.1c
T₁	5.00±0.10b	1.39±0.01ab	87.93±0.84b	3.61±0.05bc	69229 ±524.4b
T₂	5.10±0.10ab	1.37±0.02b	88.67±1.12b	3.72±0.12ab	70468 ±934.7b
T₃	5.23±0.12a	1.38±0.02ab	90.00±0.17a	3.78±0.06a	70480±849.4b
T₄	5.07±0.12ab	1.37±0.01b	88.63±0.74b	3.70±0.11ab	73566±250.9a

处理	可溶性固形物/%	可滴定酸/%	维生素C/(µg/mL)	糖酸比	产量/(kg/hm²)
CK	5.00±0.10b	1.40±0.02a	85.40±1.01c	3.56±0.10c	33939 ±2829.1c
T₁	5.00±0.10b	1.39±0.01ab	87.93±0.84b	3.61±0.05bc	69229 ±524.4b
T₂	5.10±0.10ab	1.37±0.02b	88.67±1.12b	3.72±0.12ab	70468 ±934.7b
T₃	5.23±0.12a	1.38±0.02ab	90.00±0.17a	3.78±0.06a	70480±849.4b
T₄	5.07±0.12ab	1.37±0.01b	88.63±0.74b	3.70±0.11ab	73566±250.9a