Cloning of PvLEA Gene in Snap Bean and Identification of Salt Tolerance Function

doi:10.11924/j.issn.1000-6850.casb2024-0284

Abstract

Abstract:

The aims are to meet the new challenge of soil salinization to global agriculture, explore salt tolerance genes in snap beans, and improve their salt tolerance. Using the salt tolerant variety P19023 of snap beans as the test material, the PvLEA (Phvul. 008G228100) gene was cloned and its expression characteristics were analyzed, and a PvLEA overexpression strain was created on the background of wild-type Arabidopsis thaliana Col-0. The salt tolerance related physiological indicators of the transgenic strain were identified. The results showed that PvLEA gene could be induced to express under salt stress. Compared with wild plants, PvLEA overexpression plants increased root length by 95.83%, biomass by 34.07%, SOD activity by 99.47%, POD activity by 112.58%, CAT activity by 28.57%, proline content by 811.02%, ASA content by 113.33%, MDA content by 55.4% under salt stress. These results indicated that PvLEA played a positive regulatory role in salt tolerance, which provided a theoretical basis for further study and breeding of new salt tolerant varieties of snap beans.

Key words: snap bean, salt tolerance gene, late embryogenesis abundance (LEA) protein, clone, overexpression, Arabidopsis thaliana, functional identification, ROS

LIU Chang, ZHANG Yu, LI Mengyu, FENG Guojun, LIU Dajun, YANG Xiaoxu, YAN Zhishan. Cloning of PvLEA Gene in Snap Bean and Identification of Salt Tolerance Function[J]. Chinese Agricultural Science Bulletin, 2025, 41(5): 44-49.

Figures/Tables 7

References 26

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基因名称	引物序列(5'-3')
PvLEA-F(qPCR)	CGGTGGTAAACGAAGCAACG
PvLEA-R(qPCR)	CACCACCAGAGGAAGTGACG
β-actin	CCAAGGCAAGGTACGATGAAA
β-actin	AGAGATGGGAACGAAGGGGAT

基因名称	引物序列(5'-3')
PvLEA-F(qPCR)	CGGTGGTAAACGAAGCAACG
PvLEA-R(qPCR)	CACCACCAGAGGAAGTGACG
β-actin	CCAAGGCAAGGTACGATGAAA
β-actin	AGAGATGGGAACGAAGGGGAT