Abstract:

This study aims to explore the effects of azadirachtin on the structure and function of the gut microbial community of the oriental fruit fly (Bactrocera dorsalis). Using the oriental fruit fly as the test material, the high-throughput sequencing techniques of 16S rDNA and 18S rDNA were employed to study the structure of the gut microbial community of the oriental fruit fly. The results showed that after azadirachtin treatment, the intestinal microbial community structure of B. dorsalis changed significantly. After treatment with azadirachtin, at the phylum level of gut bacteria of the oriental fruit fly, the relative abundance of Proteobacteria increased from 21.95% to 49.13%, and the relative abundance of Firmicutes decreased from 58.41% to 16.98%. At the genus level of bacteria, the relative abundance of Lactobacillus decreased from 50.96% to 5.95%. At the species level of bacteria, the relative abundance of Enterococcus durans decreased from 7.22% to 0.68%. At the phylum level of fungi, the relative abundance of Ascomycota increased from 78.95% to 95.95%, and the relative abundance of Pichia decreased from 95.41% to 78.12%. Metabolic pathway analysis showed that gut bacteria were involved in various functions such as carbohydrate metabolism, amino acid metabolism, and energy metabolism. On the whole, azadirachtin can significantly alter the structure of the gut microbial community of the oriental fruit fly, increase the diversity of bacterial colonies, disrupt the gut homeostasis, and provide new ideas for biological control.

Key words: azadirachtin, Bactrocera dorsalis, microbial diversity, intestinal flora, high-throughput sequencing