Research Progress on Plant Terpene Synthase

doi:10.11924/j.issn.1000-6850.casb2025-0106

Abstract

Abstract:

This study aims to clarify the key role of plant terpenoid synthase (TPS) in terpenoid biosynthesis and its complex regulation mechanism, and to explore its diverse biological characteristics and broad application prospects. Based on existing literature and research findings, we employed systematic review and comprehensive analysis methods to investigate the functional mechanisms and environmental response patterns of TPS from multiple dimensions: functional classification, evolution, structural catalytic characteristics, and multi-level regulation (DNA, transcription, and post-transcription). TPS catalyzes the formation of various terpenoid skeletons through its diverse structural domains, and the catalytic process is highly complex. The activity of TPS is strictly regulated by multiple levels; transcriptional regulation is mediated by various transcription factors; post-transcriptional regulation encompasses mRNA stability, translation efficiency, and protein post-translational modification. Environmental factors (e.g., biotic/abiotic stresses) have been demonstrated to significantly influence TPS gene expression and enzyme activity, thereby modulating the synthesis and accumulation of specific terpenoid products. Members of different TPS families exhibit divergence in evolution, and their functional differentiation is closely related to plant adaptability and terpenoid diversity. The relationship between the structure and function of TPS provides a molecular basis for understanding their substrate selectivity and product specificity. Research on TPS provides a core theoretical foundation for elucidating terpenoid biosynthesis and its regulatory mechanisms, significantly promoting their applications in agriculture, medicine, fragrances, cosmetics, and industry. This work holds substantial importance for sustainable development, human health, and the improvement of quality of life.

Key words: terpene synthase, structure, evolution, regulatory mechanism, application

HE Weixian, LI Xulong, ZHANG Long, GU Yan, WANG Jihua, XU Shiqiang. Research Progress on Plant Terpene Synthase[J]. Chinese Agricultural Science Bulletin, 2025, 41(34): 13-22.

Figures/Tables 2

References 88

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植物	转录因子	次生代谢产物	参考文献
黄花蒿(Artemisia annua)	AaERF1、AaERF2(AP2/ERF)	青蒿素(+)	[52]
	AaORA(AP2/ERF)	—	[53]
	AabZIP1(bZIP)	—	[54]
长春花(Catharanthus roseus)	ORCA1、ORCA2(AP2/ERF)	长春碱(+)	[55]
	ORCA3(AP2/ERF)	—	[56-59]
	CrMYC2(bHLH)	—	[60]
	CrGBF1、CrGBF2(bZIP)	—	[61]
棉花(Gossypium arboreum)	GaWRKY1(WRKY)	杜松烯(+)	[62]
拟南芥(Arabidopsis thaliana)	MYB21、MYB24(MYB)	(E)-β-石竹烯(+)	[63]
拟南芥(Arabidopsis thaliana)	MYC2(bHLH)	(E)-β-蒎烯(+)	[64]
红豆杉(Taxus wallichiana)	TcWRKY1(WRKY)	紫杉醇(+)	[65]
	TcAP2、TcDREB(AP2/ERF)	—	[66]
	TcJAMYC(bHLH)	—	[67]
水稻(Oryza sativa)	OsTGAP1(bZIP)	抗毒素(+)	[68]
水稻(Oryza sativa)	OsbZIP79(bZIP)	—	[69]
短角蒲公英(Taraxacum brevicorniculatum)	TbbZIP1(bZIP)	天然橡胶(+)	[70]

植物	转录因子	次生代谢产物	参考文献
黄花蒿(Artemisia annua)	AaERF1、AaERF2(AP2/ERF)	青蒿素(+)	[52]
	AaORA(AP2/ERF)	—	[53]
	AabZIP1(bZIP)	—	[54]
长春花(Catharanthus roseus)	ORCA1、ORCA2(AP2/ERF)	长春碱(+)	[55]
	ORCA3(AP2/ERF)	—	[56-59]
	CrMYC2(bHLH)	—	[60]
	CrGBF1、CrGBF2(bZIP)	—	[61]
棉花(Gossypium arboreum)	GaWRKY1(WRKY)	杜松烯(+)	[62]
拟南芥(Arabidopsis thaliana)	MYB21、MYB24(MYB)	(E)-β-石竹烯(+)	[63]
拟南芥(Arabidopsis thaliana)	MYC2(bHLH)	(E)-β-蒎烯(+)	[64]
红豆杉(Taxus wallichiana)	TcWRKY1(WRKY)	紫杉醇(+)	[65]
	TcAP2、TcDREB(AP2/ERF)	—	[66]
	TcJAMYC(bHLH)	—	[67]
水稻(Oryza sativa)	OsTGAP1(bZIP)	抗毒素(+)	[68]
水稻(Oryza sativa)	OsbZIP79(bZIP)	—	[69]
短角蒲公英(Taraxacum brevicorniculatum)	TbbZIP1(bZIP)	天然橡胶(+)	[70]