Abstract:

The study investigated the impact of konjac continuous cropping on the microbial community and relevant functional genes in the rhizosphere soil, providing a theoretical basis for addressing the continuous cropping obstacle in the konjac cultivation. Through metagenomic sequencing technology, the characteristics of microbial community were analyzed in konjac rhizosphere soil samples from the fields that never been planted with konjac (Mock), planted in the current year (SC1Y), and continuously planted for 13 years (SC13Y), respectively. The abundance and function of microorganisms and soil enzymes were annotated using the NR, eggNOG, KEGG, and CAZy databases. KEGG and EggNOG functional predictions indicated that long-term continuous cropping of konjac reduced the abundance of metabolic pathways and amino acid metabolism, thereby decreasing the overall metabolic capacity of soil microorganisms. Based on CAZy annotation analysis, glycoside hydrolases and glycosyltransferases were the most abundant in the soils under long-term continuous cropping, leading to a reduction in carbohydrates in the konjac rhizosphere soil. Significant differences were observed in the microbial composition of konjac rhizosphere soils under different cropping durations, and long-term continuous cropping altered the functional structure of the microbial community in the konjac rhizosphere soil.

Key words: Amorphophallus konjac, rhizosphere soil, continuous cropping, metagenomics, microbiology community structure and function