Effects of Combined Application of Bio-organic Fertilizer on Yield of Industrial Hemp and Its Rhizosphere Soil Microbial Structure

doi:10.11924/j.issn.1000-6850.casb2025-0435

Abstract

Abstract:

To clarify the effect of combined application of bio-organic fertilizer on the rhizosphere soil microbial community of industrial hemp, metagenomic sequencing technology was used to compare and analyze the effects of different treatments of fallow land (CX), no fertilization (CB), single application of chemical fertilizer (FH), and combined application of 20% bio-organic fertilizer (FS) on the composition and structure of rhizosphere soil microbial communities of industrial hemp. The results showed that the combined application of bio-organic fertilizer increased the contents of available phosphorus (AP), available potassium (AK), alkaline hydrolyzable nitrogen (AN), total nitrogen (TN), total phosphorus (TP), total potassium (TK) and soil organic matter (SOM) in the rhizosphere soil of industrial hemp by 9.01%-79.41%, while reducing soil pH by 1.51%-4.16%. The combined application of bio-organic fertilizer had a regulatory effect on the rhizosphere microorganisms of industrial hemp; after application, the relative abundances of Actinobacteria, Planctomycetes, Sphingomonas, Nitrospira and Afipia increased. PCA analysis indicated a trend of separation in microbial communities among soil samples under different treatments, with significant differences in bacteria, fungi, and archaea between the combined bio-organic fertilizer treatment and other treatments. For the influence of soil factors on microbial community structure under different treatments, the order was AK>TN>AN>SOM>AP>TP>TK>pH. At the phylum level, the influence of soil chemical properties on microbial communities could be divided into two groups: pH alone formed one group, while other chemical properties had similar effects on soil microbial community structure at the phylum level. It was notable that Chloroflexi showed a significant negative correlation with pH. The combined application of bio-organic fertilizer increased the yield of industrial hemp and the nutrient content in rhizosphere soil, improved the soil microbial community structure, enhanced the quantity and diversity of soil microorganisms, and improved nutrient supply and transformation capacity. This study provided a theoretical basis for black soil remediation in Northeast China.

Key words: industrial hemp, yield, soil, bio-organic fertilizer, microbial structure, microbial diversity

ZHANG Xiaoyan, CAO Kun, LI Xuehai, ZHU Hao, BIAN Jing. Effects of Combined Application of Bio-organic Fertilizer on Yield of Industrial Hemp and Its Rhizosphere Soil Microbial Structure[J]. Chinese Agricultural Science Bulletin, 2025, 41(34): 87-95.

Figures/Tables 10

References 29

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处理	AN/(mg/kg)	AP/(mg/kg)	AK/(mg/kg)	TN/(g/kg)	TP/(g/kg)	TK/(g/kg)	pH	SOM/%
CX	71.74d	13.85d	102.30d	0.96d	0.20d	20.73d	8.20a	2.01c
CB	88.59c	15.25c	106.85c	1.03c	0.23c	21.86c	7.98ab	2.10c
FH	114.86b	24.56b	143.45b	1.31b	0.27b	22.78b	8.09ab	2.14b
FS	129.75a	27.41a	156.50a	1.50a	0.36a	24.85a	7.86b	2.33a

处理	AN/(mg/kg)	AP/(mg/kg)	AK/(mg/kg)	TN/(g/kg)	TP/(g/kg)	TK/(g/kg)	pH	SOM/%
CX	71.74d	13.85d	102.30d	0.96d	0.20d	20.73d	8.20a	2.01c
CB	88.59c	15.25c	106.85c	1.03c	0.23c	21.86c	7.98ab	2.10c
FH	114.86b	24.56b	143.45b	1.31b	0.27b	22.78b	8.09ab	2.14b
FS	129.75a	27.41a	156.50a	1.50a	0.36a	24.85a	7.86b	2.33a

处理	observed_species	ACE	Chao1	Shannon	Simpson	goods_coverage	Pielou_evenness
CX	13756.33bc	13831.39ab	13784.51ab	4.58bc	0.89bc	1.00a	0.48b
CB	14076.67ab	14152.49ab	14104.16ab	4.66ab	0.90ab	1.00a	0.49ab
FH	14278.00ab	14359.05ab	14308.55ab	4.77ab	0.90ab	1.00a	0.50ab
FS	15136.00a	15183.39a	15153.45a	4.92a	0.92a	1.00a	0.52a

处理	observed_species	ACE	Chao1	Shannon	Simpson	goods_coverage	Pielou_evenness
CX	13756.33bc	13831.39ab	13784.51ab	4.58bc	0.89bc	1.00a	0.48b
CB	14076.67ab	14152.49ab	14104.16ab	4.66ab	0.90ab	1.00a	0.49ab
FH	14278.00ab	14359.05ab	14308.55ab	4.77ab	0.90ab	1.00a	0.50ab
FS	15136.00a	15183.39a	15153.45a	4.92a	0.92a	1.00a	0.52a