Abstract: Substrate experiment was carried out to study the effects of different nitrogen levels :N1 ( 6mmo1/L ), N2 ( 10mmo1/L ), N3 (14mmol/L ), N4(18mmol/L ), N5(22mmol/L )on the Nitrate accumulation and nutriment quality in different parts in amaranth. The results showed that the content of nitrate increased with the increasing of nitrogen levels, and linear correlation, soluble sugar and the protein decreased. the vitamin C and the organic acid increased firstly then softly decreased, but the vitamin C do not have the obvious change. The optional nitrogen level for amaranth growth was 14mmol/L.