Abstract: 【OBJECTIVE】To investigate the effect of five kinds of fertilizers, manures of man, pig and duck, chemical fertilizer and conventional fertilizer, at three application levels on the growth of Houttuynia cordata Thunb.【METHOD】Using the Houttuynia cordata Thunb mainly cultivated in the base of GAP in Xiaohu Jianyang, Fujian province, a single factor randomized block design with the five fertilizers at three application levels was employed to investigate enzyme activities of the rhizospheric soil and root vigors in the plant.【RESULTS】The application of organic fertilizers were contributed to increase the activities of urease, proteinase and cellulase in soil enzyme system. Otherwise, the application of chemical fertilizers enhance the activities of saccharase and phosphatase, as while, to enhance its root vigors in the appalication of organic and conventional fertilizer. The activities of urease was the highest at the treatment with 72000 kg/hm2 mature of human (296.8 mgNH3-N/g DW), the activities of proteinase was the highest at the treatment with 36000 kg/hm2 mature of duck (5.15 mg NH2-N/g DW), the activities of cellulase was the highest at the treatment with 36000 kg/hm2 pig mature (0.869 mg glucose/g DW), the activities of saccharase and phosphatase were both the highest at the treatment with 1980 kg urea, 3600 kg/hm2 Ca3(PO4) and 1305 kg/hm2 K2SO4, having the value of 13.10 mgglucose/g DW and 44.46 mg P2O5/100g DW respectively. 【CONCLUSION】 The application of organic fertilizers increased the activities of urease, proteinase and cellulase in the rhizospheric soil and root vigors in the plant, which may promote the process of developmental growth and the formation of yield in Houttuynia cordata.