Abstract: In order to learn the relationship of peroxidase diversity with F1 Yield in different hybrids, 6 different hybrids were employed to evaluate the yield performance with peroxidase diversity in this investigation. The results showed that peroxidase diversity was related to F1 Yield in 6 different hybrids. It showed that the yield heterosis had correlation with the numbers of parents. F1 Yield performance was calculated based on peroxidase diversity in order to analyze relationship. The heterozygosity of peroxidase can be used as an index in predicting the yield heterosis of maize.