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Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (2): 89-089.

Special Issue: 生物技术

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DNA Extraction and Optimization of RAPD Reaction System for Lilium Asiatic hybrids (Liliaceae)

Zhao Chen   

  • Online:2007-02-05 Published:2007-02-05

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Abstract: In order to accelerate the molecular biology research of Lilium asiatic hybrids, DNA of Lilium asiatic hybrids was extracted from the fresh leaves by CTAB. The optimized reaction system for Lilium asiatic hybrids are as follows. The reactions were performed in a volume of 25 μl containing 2.0 mmol/L Mg2+, 160 μmol/L dNTPs, 1.5 U Taq DNApolymerase, 0.3 μmol/L primer.

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