Abstract: The regeneration way of bud clumps was adopted to establish a rapid technique system of propagation in Oncidium in this experiment. The main contents focus on the following aspects, such as choice of basic mediums, combination and density of hormone and addictions. The Orthogonal Design is adopted to arrange experiment. Through above research, it was tried to find the main factors and improve the propagation coefficient and build up the superior regeneration system in Oncidium in the paper. The result showed that the main factors of multiplication of bud clumps are the basic medium, BA and NAA. The best multiplication medium is MS + BA 3.0 or 4.0 mg/L + NAA 0.4 or 0.6 mg /L + coconut milk 150.0 ml/L or tomato juice 150.0 ml /L + active carbon 1.0 g /L + Vc 0.2 mg /L + active carbon 1.0g /L + sugar 30.0 g /L + agar 8.0g /L.