Abstract: The effects of different matrixes on the growth and flowering of the young and matured seedlings of tissue culture of Rhynchostylis gigantea are studied respectively, to solve the selection problem of the seedling cultivation matrix during the different production stage. The results were as follows: (1) sphagnum was a suitable cultivation matrix for young seedling. The survival rate of greenhouse and shade shed environment was 97.5%, which can promote plant growth better. The survival rate of pine squama treatment was 100.0% and 90.0%, respectively. It has a better promotion effect and is sub-optimal treatment. (2) The mixed matrix of pine bark, charcoal and volcanic rocks was beneficial to the growth of leaves and roots of the matured seedlings, which was a suitable cultivation matrix for the vegetative growth period but was not conducive to flowering. The flowering rate of 1.0-2.0 cm volcanic rock and pine squama was 90.0% and 75.0% respectively. It has a certain promotion effect on leaf width, stem height and root coarse growth, which was suitable for the flower cultivation of matured seedling. Among them, pine squama is more economical and environmentally friendly, and is preferred flower-promoting matrixes.