Abstract: 【OBJECTIVE】Fast identification and detection of Cucumber green mottle mosaic virus (CGMMV）is the key way to control its further spreading.【METHOD】 To establish procedure of identification and detection, mechanical inoculation, transmission electron microscopy, double antibody sandwich ELISA (DAS-ELISA) and reverse transcription polymerase chain reaction (RT-PCR) were applied in this study. 【RESULTS】CGMMV was detected in a cucumber sample which collected from a greenhouse in Nanning city , Guangxi province. By optimizing the RT-PCR reaction system and amplification procedures, immuno-capture RT-PCR（IC-RT-PCR）method was then established. 【CONCLUSION】The results showed that IC-RT-PCR is not only simplify the extraction of RNA but also decline the demand of sample. IC-RT-PCR is specific, simple and convenient, and could be used as a rapid and efficient detection method for CGMMV diagnosis.