Abstract: Creation and utilization of high oil soybean germplasm resources is benefit for accelerating breeding progress, shortening years, increasing peasants’ income and promoting oil corporation revenue. Using the genetic effect of recombination, accumulating, complement and mutation, the cross or multiple cross between high oil soybean that had been selected and appraised and fine varieties introduced from domestic and foreign were carried out, then the quality of F6 was analyzed. The new variety that contented oil more than 22.5% was identified and generalized. It improves the genetic composition and broad soybean blood relationship. And it is also important for breeding high oil soybean.