Abstract: SSR primers were developed from the 24 cultivars of sweet cherry such as ‘Hongdeng’, ‘Summit’, ‘Jisaila 5’, ‘Jisaila 6’ by 2.5% agar gel electrophoresis. The aim is to select the SSR markers with significant selective difference and a few alleles (2 alleles). The SSR primers, which can amplify stable and distinct band type between different materials, were selected from the 38 pairs of primers, and 10 primer pairs were used to analyze the fingerprint of sweet cherry. The electrophoresis results of the 24 sweet cherry DNA samples were evaluated and analyzed with the softwares of Visual Basic and Access, and the database was established. The amplified bands of the selected 10 primer pairs were lined up, which became the gene identification number of sweet cherry. Eventually, the sweet cherry germplasms were identified according to their molecular identification.