Abstract: Seventy-five milk cows that cause acute mammitis were divided into three groups randomly and different therapeutic methods were adopted. Result is indicated: efficiency and the curative ratio reaches 100% equally if we mixed traditional Chinese medicine and western medicine. Using penicillin and streptomycin to treat, the efficiency is 92% and the curative ratio is 88%. Efficiency is 84% and curative ratio is 80% if we only use traditional Chinese medicine. Penicillin and streptomycin mixing traditional Chinese medicine has complementary advantages and the cure effect is the best. It is worth to apply this method to cure acute mammitis of milk cow.