Abstract: 【Objective】To clone and analysis the porcine musclin.【Method】Total RNA was extracted from muscle tissue and mRNA sequence of gene were amplified by RT-PCR using two designed primers. The PCR products were ligated into the pMD-19Tvector, and then transformed into competent cells of E. coli DH5α.The sequence was analyzed to identify the recombinant plasmid.【Results】Identity analysis showed that the musclin nucleotide sequence shared 86%, 78% and 75% homology with that of human , rat and mouse. The predicted peptide contained a “KKKR” motif and the region homologous to mouse ANP, BNP, and CNP.【Conclusion】The porcine partial musclin gene was successfully cloned in present study and the sequence has been submitted to GenBank(Accession.EF369511).