Primary Study of Function of A Rice WRKY19 Gene Promoter

Abstract

Abstract: Using transgenic plants of rice WRKY19 gene promoter (OsW19p::GUS) to do this experiment, the inducible expression characteristic of OsW19p in T1 seedlings, detected by GUS fluorometric measurements revealed that: expression level of OsW19p was increased by ABA(100μmol/L),SA(500μmol/L), high temperature(42℃), low temperature (5℃) and wounding treatments; And was repressed by 2,4-D(1 μmol/L), NaCl(200 mmol/L)and PEG4000(25%) treatments; IAA(5 μmol/L), MeJA(100 μmol/L)and ultraviolet treatments can’t influence expression. Expression level of roots of OsW19p was higher than 35s in transgenic plants, but leaves of it was lower one. Furthermore, expression level of clums of transgenic plants was the highest in the flower stage, followed by flowers, sheathes and leaves, the roots showed the lowest expression.

CLC Number:

S435

Hao Zhongna, Wang Haihua, Wu Xuelong, Guo Zejian. Primary Study of Function of A Rice WRKY19 Gene Promoter[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 99-99.

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