Genetic Diversity Analysis of Onion (Allium cepa L.) Germplasm by ISSR Markers

Abstract

Abstract: The genetic diversity of 32 onion (Allium cepa L.) germplasm resources were analyzed by ISSR（inter-simple sequence repeat）. Four ISSR primers were screened from 31 ISSR primers. The four ISSR primers generated 39 clear bands, which 31 were polymorphic, averaged 7.75 bands each primer pair. The average percentage of polymorphic bands was 79.48 %. The genetic similarity ranged from 0.552 to 0.960, which indicated a rich genetic diversity of onion (Allium cepa L.) germplasm resources. According the result of UPGMA cluster analysis based on ISSR makers, the tested materials could be divided into five groups at the level of D=0.68. First group including 18 germplasm materials is the Yellow sweet Spanish system. Second group including one germplasm material is Bejo Dyatona cultivar. Third group including 3 germplasm materials is the Yellow Globe system. Fourth group including 9 germplasm materials is the Yellow Globe Danvers system. Fifth group including one germplasm material is the Yellow Danvers system. The results indicated that ISSR technique is useful in revealing the genetic diversity and genetic relationship among onion germplasm materials, providing a scientific basis for genetic breeding and patents selection.

CLC Number:

S633.2

Xu Qijiang, Cui Chengri, Jia Tiejin. Genetic Diversity Analysis of Onion (Allium cepa L.) Germplasm by ISSR Markers[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 126-126.

References

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