Cloning and expression of s-adenosylmethionine synthetase of sweetpotato

Abstract

Abstract: In order to explore the relationship between cold stress and the change of gene expression. Suppression subtractive hybridization (SSH) method was used to identify novel genes regulated by cold stressed in sweetpotato. A full length cDNA coding region of S-Adenosyl-L-Methionine synthase (SAMS) which contains 1182bp was isolated by this way combined with RT-PCR technology. Sequence analysis revealed that this genes hared 85.48 % similarity in gene sequence and 93.6 % similarity in protein sequence with the other SAMS gene in plant. The differences of nucleotides sequences were higher than those of amino acids. The phylogenetic tree was constructed based on the alignment nucleotides sequences elucidated the relationship among different plant species at nucleotide and amino acids level, which could proved some help for the utilization of plant germplasms. Fused expression plasmid vector pET-SAMS was constructed with sams and pET32a. A protein about 63KDa was expressed after 3h induced by IPTG at different temperature in BL21.

CLC Number:

Cloning and expression of s-adenosylmethionine synthetase of sweetpotato. Cloning and expression of s-adenosylmethionine synthetase of sweetpotato[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 121-121.

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