Effect of Different Promoters on Expression of HA-VP2 Recombinant Baculovirus Protein

doi:10.11924/j.issn.1000-6850.casb16120133

Abstract

Abstract: In order to investigate the effect of different promoters of the expression of IBDV-VP2 protein to chose the better expression system. In this research, HA-TAG was added to the epitope of IBDV-VP2 gene, which was integrated into the 5 "end of VP2 gene. The HA-VP2 gene was amplified and integrated into the recombination transfer vector containing CMV, CBA and EF1α promoters Vector to construct HA-VP2 recombinant baculovirus. The Chinese Hamster Ovary (CHO) cells were infected with the recombinant baculovirus, and the optimal promoter was determined by bioinformatics software. The results showed that CMV promoter was similar to CBA promoter activity and significantly different from EF1α promoter activity (p <0.05). And this study will provide a new idea for the development of avian genetic engineering vaccines by comparing the effects of different promoters on the protein expression level.

CLC Number:

Q786

Yu Hang,Gao Dongni,Tian Zhaofeng,Ping Wenxiang and Ge Jingping. Effect of Different Promoters on Expression of HA-VP2 Recombinant Baculovirus Protein[J]. Chinese Agricultural Science Bulletin, 2017, 33(23): 100-105.

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