AFLP Analysis of 7 SH Line Apple Rootstocks

Abstract

Abstract: The improved method of CTAB-DNA isolation was used to extract genomic DNA from leaves of 7 SH lines (Malus domestica Borkh. X Malus. Honanensis Rehder), which are used as apple rootstocks. The materials were analyzed by AFLP (Amplified Fragment Length Polymorphism)-silver staining protocol in order to detect blood relationship and genetic diversity. 20 pairs primers were selected from 143 primer pairs(3+3)to amplify the genetic DNA. Total 458 distinct amplification bands were obtained, 261 of them were polymorphic markers and the polymorphic frequency was 57%. UPGMA cluster analysis based on simple matching coefficient (SM) divided the 7 SH line apple rootstocks into 2 groups at 0.58 level.

CLC Number:

S661

Pu Nana, Du Guoqiang, Li Mingyuan, Zhang Jianwen, Ma Baokun. AFLP Analysis of 7 SH Line Apple Rootstocks[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 141-141.

[1]	LI Tingting, CHEN Hong. Study on Callus Induction and Anti-browning of Rosa roxburghii Tratt Leaves [J]. Chinese Agricultural Science Bulletin, 2022, 38(32): 12-21.
[2]	ZHAO Mingxin, CAO Gang, WANG Wei, CAO Sufang, LI Hongxu. Comparative Study on Pollination Effects of Five Pear Varieties for ‘Yulu Xiangli’ Pear [J]. Chinese Agricultural Science Bulletin, 2022, 38(28): 52-57.
[3]	QIAO Xuqiang, GUO Tingting, YANG Bingsong, LI Jianzhao, LIANG Meixia. Anatomical Structure Changes of Roots, Stems and Leaves of Apple Plantlets in Vitro During Transplanting [J]. Chinese Agricultural Science Bulletin, 2022, 38(22): 49-54.
[4]	ZENG Shaomin, CHEN Xiaoming, JIANG Cuicui, HUANG Xinzhong. Potential Adverse Meteorological Factors in Main Phenological Periods of Pear Under Year-round Rain-shelter Cultivation Without Skirt Film [J]. Chinese Agricultural Science Bulletin, 2022, 38(22): 99-103.
[5]	WANG Guiping, XUE Xiaomin, ZHAO Hongqiang, CHEN Ru, HAN Xueping, WANG Jinzheng. Effects of No-Bagging and Bagging Density on Photosynthetic Characteristics of ‘Fuji’ Apple Trees [J]. Chinese Agricultural Science Bulletin, 2022, 38(13): 54-59.
[6]	PENG Cheng, DENG Linping, MENG Wancong, CHANG Xiaoxiao, ZHANG Zengwen, LUO Jianliang, QIU Jishui, LU Yusheng. Effect of Bagging on Fruit Appearance and Aromatic Components of Prunus salicina var. cordata [J]. Chinese Agricultural Science Bulletin, 2022, 38(7): 45-51.
[7]	YU Huili, XU Bianbian, XU Guoyi, SHAO Wei, GAO Dengtao, SI Peng. Effects of Bio-organic Fertilizer on Growth, Physiological Characteristics of Apple Seedlings and Soil Microbial Functional Diversity [J]. Chinese Agricultural Science Bulletin, 2022, 38(1): 32-38.
[8]	ZHANG Zishen, KE Zehua, ZHAO Shuliang, CHENG Fuhou, LI Xiangxi. Effect of Harvest Period on the Quality of ‘Huangguan’ Pear [J]. Chinese Agricultural Science Bulletin, 2022, 38(1): 39-43.
[9]	Chen Yongfa, Zhou Hui, Song Liubin, Fan Cheng, Wang Yuefeng. Effects of Organic Fertilizer and Microbial Inoculants on the Yield and Quality of Xinning Navel Orange [J]. Chinese Agricultural Science Bulletin, 2021, 37(35): 38-42.
[10]	Zhang Feng, Jiang Zhiqin, Chen Xiaoguang, Li Shiqiang, He Tianming. The Development Factors of Pyrus sinkiangensis ‘Korla Xiangli’ Industry [J]. Chinese Agricultural Science Bulletin, 2021, 37(34): 159-164.
[11]	Duan Pengwei, Ma Xiaojian, Shi Haiqiang, Du Jizhuang, Qin Lizhe, Xu Guoliang, Yang Sumiao. Correlation Analysis of Fruit Quality of ‘Fuji’ Apple and Altitude [J]. Chinese Agricultural Science Bulletin, 2021, 37(22): 49-56.
[12]	Peng Cheng, Meng Wancong, Deng Linping, Chang Xiaoxiao, Luo Jianliang, Chen Zhe, Qiu Jishui, Lin Zhixiong, Lu Yusheng. Effects of Transformation on Soil Nutrients and Fruit Quality of ‘Xinghuayin’ Pear Orchard [J]. Chinese Agricultural Science Bulletin, 2021, 37(21): 40-45.
[13]	Wu Xiaoxuan, Wang Hongning, Sun Junbao, Zhang Shengzhi. Effects of Soil Natural Vegetation Management Models on the Soil and Apple Tree of Dry Land Orchard [J]. Chinese Agricultural Science Bulletin, 2021, 37(19): 119-123.
[14]	Chen Qiliang, Fan Jing, Yang Xiaoping, Zhang Jingguo, Du Wei, Hu Hongju. The Diagnosis and Evaluation of Nutritional Status of Pear Leaves [J]. Chinese Agricultural Science Bulletin, 2021, 37(19): 52-57.
[15]	Yang Jian, Li Kun, Wang Long, Wang Suke, Su Yanli, Xue Huabai. Ecological Adaptability and Cultivation Area of Pyrus communis in China [J]. Chinese Agricultural Science Bulletin, 2021, 37(16): 97-101.

AFLP Analysis of 7 SH Line Apple Rootstocks

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

For authors

For reviewers

Reader center

Contact us