Abstract: The β-glucan was extracted from oat in South of Shanxi province by the process of comminution, hydrolysis of starch, immersion in alkali soluble et al. The results showed that the optimum conditions were 60 hole oat powder, ratio of oat powder and water 1:10, addition of α-amylase 40U/ml, time for hydrolysis 2h, temperature for extraction 75℃, and pH8.0, dialysis for microhole film 2h. The percentage extraction of β-glucan from oat was 72.4%~95.2%.