Abstract: Samples were collected from 39 cases of cervus hemorrhagic enteritis in Shanxi province, the major microbial pathogen were isolated and examined microscopically, then identified through serum reaction, the preliminary result showed it is type A Clostridium perfringens, the result were further　confirmed by PCR and sequence analysis. So the conclusion was made that type A Clostridium perfringens is the major pathogen that causing cervus hemorrhagic enteritis in Shanxi province, and therefore provide basis for the disease prevention and vaccine development.