Abstract: With the materials of super-hybrid rice “Liang you pei jiu” and female parent Peiai64S and male parent medial duration Indica rice 9311, effects of chilling and high light on chlorophyll fluorescence parameters and photosynthetic characteristics were studied. Results showed that after treatment of chilling temperature and high light, as comparison with its parents, the decrease of net photosynthetic rate, PSⅡprimary photochemical efficiency (Fv/Fm) and photochemical quenching coefficient (qP) and the increase of nonphotochemical quenching coefficient (qN) in super-hybrid rice Liangyoupeijiu were less, which suggested that more light energy absorbed by “Liang you pei jiu” was converted into chemical energy under chilling temperature and high light. Results demonstrated that under chilling temperature and high light super-hybrid rice “Liang you pei jiu” surpassed its parents and adopted from its female parent.