Abstract: The Production of Ganoderma lucidum by solid-state fermentation using corn as substrate was studied , and the fermentation technique was optimized by single factor and orthogonal tests. The content of polysaccharide,reducing sugar,protein and amino acid were determined.The results showed that the size of crush granule, strain, time, temperature and inoculum size had significant effect on the content of polysaccharide,reducing sugar,protein and amino acid. The optimum fermentation conditions were 10 mesh granularity, 12% inoculum size, 28℃ fermentation temperature, 20d fermentation time. The content of polysaccharide was 21.97 mg/g under the optimized cultivation.