Method Analysis of Producing Transgenic Chicken Mediated by Retrovirus

Abstract

Abstract: With the fast development of Biopharmacy,transgenic chicken is growing to be one hot spot of science study in the biology field. In order to build and optimize the technology and conditions of transgenic chicken platform produced by retrovirus, pHIT-Myc3 and enhanced green fluorescent protein（EGFP）gene were selected as vector and marker gene respectively in this experiment.Vesicular stomatitis virus G glycoprotein(VSV-G) pseudotyped retrovirus was packaged by co-transfecting method.Chicken fetal myoblasts(CFM) were infected and new laying zygotes were injected with concentration retrovirus and hatched. Genomic DNA of CFM,5d chicken embryos and different tissues of new-born chicken were extracted for PCR identification.Marker gene GFP was detected from CFM, 5d chicken embryos and thymus gland and skin of new-born chicken,which indicated transgenic chicken had been produced by retrovirus successfully.At the same time,the method and conditions of producing transgenic chicken had been optimized effectively,which provides references for further investigating transgenic chicken in the future.

CLC Number:

S831.2

Man Chaolai, Chen Yan. Method Analysis of Producing Transgenic Chicken Mediated by Retrovirus[J]. Chinese Agricultural Science Bulletin, 2007, 23(8): 1-1.

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