Abstract: 【Objective】To clone and analysis the ovine regakine-1.【Method】Total RNA was extracted from mesenteric lymph node and mRNA sequence of gene were amplified by RT-PCR with designed primers. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of JM109. The recombinant plasmid was identified and the sequence was analyzed.【Results】Identity analysis showed that the regakine-1 nucleotide sequence shared 91% homology with that of cattle. The signal peptide of predicted amino acid sequence is 1~21aa and the structural domain SCY is 29~87aa, revealing that the structural feature is consistent with cattle regakine-1.【Conclusion】The ovine ORF of regakine-1 gene was successfully cloned in present study and the sequence has been submitted to GenBank(Accession. EF617337).