Abstract: 【OBJECTIVE】In order to select out the optimum wheat (Triticum aestivum L.) genotype for tissue culture of mature embryo (MEs) and receptor of genetic transformation, 【METHOD】27 winter-type superior cultivars wheat of Chinese northern region or breeding lines of hexaploid common wheat were used as test material and the method of callus induction of endosperm-supported was utilized in this paper. The effect of many factors on tissue culture of wheat MEs had been studied. 【RESULTS】The results indicated that, light, temperature, concentration of 2,4-D and genotype were important influence factors for tissue culture of wheat MEs. The genotype dependency was strongly high in tissue culture of Mes of these genotypes. Differences of frequency of induction, differentiation and regeneration of callus from mature embryos of 27 wheat genotypes by endosperm-supported method were respectively significant (P<0.01). The efficiency of induction, differentiation and regeneration of mature embryos callus was high in genotypes screened out. Mature embryo-derived callus of SN2618, TS021, HB341 showed better tissue culture response than other genotypes. Their frequency of regeneration were 15.38%, 25.27%, 21.33% respectively. The regenerated plants could be obtained in 30~40 d. 【CONCLUSION】The work provided a solid basis for tissue culture of wheat Mes, genetic transformation and molecular plant breeding of wheat.